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Int J Syst Evol Microbiol 56 (2006), 593-597; DOI  10.1099/ijs.0.63278-0
© 2006 International Union of Microbiological Societies

New Leptospira serovar Sokoine of serogroup Icterohaemorrhagiae from cattle in Tanzania

G. F. Mgode1, R. S. Machang'u1, M. G. Goris2, M. Engelbert2, S. Sondij2 and R. A. Hartskeerl2

1 Sokoine University of Agriculture, Pest Management Centre, PO Box 3110, Morogoro, Tanzania
2 Royal Tropical Institute, Department of Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands

Correspondence
G. F. Mgode
gfmgode{at}hotmail.com

The prevalence of leptospirosis is generally high in domestic animals and rodents in Tanzania. Identification of Leptospira isolates from cattle was carried out to establish prevalent Leptospira serovars. Serological typing was done based on monoclonal antibodies and the standard cross-agglutination absorption test. Molecular typing involved pathogenic- and saprophytic-specific PCRs and a PCR specifically amplifying DNA from the species Leptospira kirschneri. DNA fingerprinting with primers derived from sequences of insertion elements IS1500 and IS1533 was carried out. Both serological and molecular characterization indicated that one of the Leptospira isolates, coded RM1, represents a new serovar of the species L. kirschneri of serogroup Icterohaemorrhagiae. The serovar name Sokoine is proposed for this new Leptospira isolate.

Abbreviations: CAAT, cross-agglutination absorption test; DF, dark-field






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Copyright © 2006 by the International Union of Microbiological Societies.