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Proposal of Giesbergeria voronezhensis gen. nov., sp. nov. and G. kuznetsovii sp. nov. and reclassification of [Aquaspirillum] anulus, [A.] sinuosum and [A.] giesbergeri as Giesbergeria anulus comb. nov., G. sinuosa comb. nov. and G. giesbergeri comb. nov., and [Aquaspirillum] metamorphum and [A.] psychrophilum as Simplicispira metamorpha gen. nov., comb. nov. and S. psychrophila comb. nov.

Margarita Grabovich^1,, Ekaterina Gavrish^2,,, Jan Kuever^3,, Anatoly M. Lysenko⁴, Daria Podkopaeva¹ and Galina Dubinina⁴

¹ Department of Biochemistry and Plant Physiology, Faculty of Biology, Voronezh State University, Voronezh, Russia
² All Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia
³ Max-Planck-Institute for Marine Microbiology, Celsiusstraße 1, D-28359 Bremen, Germany
⁴ S. N. Winogradsky Institute of Microbiology of the Russian Academy of Sciences, Pr. 60-letiya Octyabrya 7/2, Moscow, 117811, Russia

Correspondence
Margarita Grabovich
margarita_grabov{at}mail.ru

Five Gram-negative, motile, spiral-shaped strains were isolated from a sulfide spring (D-412^T), active sludge of wastewater (D-419^T, D-420, D-424) and industrial wastewater (D-416). Comparative 16S rRNA gene sequence analysis showed that the isolates belong to the family Comamonadaceae, within the class Betaproteobacteria, but fall into a distinct cluster. On the basis of phenotypic, chemotaxonomic and phylogenetic data, a new genus, Giesbergeria gen. nov., is proposed, including five species. The type species of the genus is Giesbergeria voronezhensis sp. nov. (type strain D-419^T=DSM 12825^T=CIP 107340^T=VKM B-2350^T) and other novel members of the genus are Giesbergeria kuznetsovii sp. nov. (type strain D-412^T=DSM 12827^T=VKM B-2352^T), Giesbergeria giesbergeri comb. nov. (basonym Aquaspirillum giesbergeri), Giesbergeria sinuosa comb. nov. (basonym Aquaspirillum sinuosum) and Giesbergeria anulus comb. nov. (basonym Aquaspirillum anulus). Using the same criteria, isolate D-416 (=DSM 12826) was identified as a strain of [Aquaspirillum] metamorphum. Strain D-416, the type strain of [A.] metamorphum and the type strain of [Aquaspirillum] psychrophilum form a distinct cluster within the family Comamonadaceae (97–97·2 % 16S rRNA gene sequence similarity) and share phenotypic and chemotaxonomic properties. Therefore, it is proposed that these strains are reclassified as members of a new genus, Simplicispira gen. nov., as Simplicispira metamorpha comb. nov. (the type species) and Simplicispira psychrophila comb. nov., respectively.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains D-419^T, D-420, D-412^T and D-416 are AY780905–AY780907 and AY780904, respectively.

Tables detailing the strains used in this study, the fatty acid profiles of strains and the DNA–DNA relatedness between strains and images showing the morphology of Giesbergeria voronezhensis sp. nov. and Giesbergeria kuznetsovii sp. nov. are available as supplementary material in IJSEM Online.

These authors contributed equally to the work.

Present address: Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.

Present address: Bremen Institute for Materials Testing, Foundation Institute for Materials Science, Paul-Feller-Straße 1, D-28199 Bremen, Germany.

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