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State Key Laboratory of Microbial Resources at the Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, PR China
Correspondence
Shuang-Jiang Liu
shuangjiang{at}hotmail.com
Actinomycete strain H9T was isolated from coastal sediment of the Jiao-Dong peninsula (near Tsingdao city) in China, and was identified by means of polyphasic taxonomy. The strain grew autotrophically on modified nitrifying medium and heterotrophically on LuriaBertani medium, with NaCl ranging from 0 to 8 % (w/v) (optimal growth at 3·5 %). The 16S rRNA gene sequence similarities of strain H9T to members of the genus Pseudonocardia ranged from 93·0 to 97·5 %, indicating that strain H9T was phylogenetically related to members of the genus Pseudonocardia. Strain H9T had type IV cell wall and type PIII phospholipid, and its major menaquinone was MK-8 (H4). DNADNA relatedness values between strain H9T and Pseudonocardia kongjuensis, Pseudonocardia autotrophica and Pseudonocardia compacta were 42, 13 and 11 %, respectively. These results support the conclusion that H9T represents a novel species within the genus Pseudonocardia, for which the name Pseudonocardia ammonioxydans sp. nov. is proposed, with the type strain H9T (=CGMCC 4.1877T=JCM 12462T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain H9T is AY500143.
A figure showing an electron micrograph of strain H9T and tables showing ammonia oxidation of nitrite and nitrate and cellular fatty acid composition of strain H9T are available as supplementary material in IJSEM Online.
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