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Methylocystis rosea sp. nov., a novel methanotrophic bacterium from Arctic wetland soil, Svalbard, Norway (78° N)

Ingvild Wartiainen^1,, Anne Grethe Hestnes¹, Ian R. McDonald² and Mette M. Svenning¹

¹ Department of Biology, Faculty of Science, University of Tromsø, N-9037 Tromsø, Norway
² Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton, New Zealand

Correspondence
Mette M. Svenning
Mette.Svenning{at}ib.uit.no

A Gram-negative, rod-shaped, non-motile, non-spore-forming, pink-pigmented bacterium, SV97^T, was isolated from a wetland soil near Ny-Ålesund, Svalbard Islands, Norway (78° N). On the basis of 16S rRNA gene sequence similarity, strain SV97^T was shown to belong to the Alphaproteobacteria and was highly related to a number of non-characterized Methylocystis strains with GenBank accession nos AJ458507 and AJ458502 (100 %) and AF177299, AJ458510, AJ458467, AJ458471, AJ431384, AJ458475, AJ458484, AJ458501 and AJ458466 (99 %). The most closely related type strains were Methylocystis parvus OBBP^T (97·2 %) and Methylocystis echinoides IMET 10491^T (97 %). The closest related recognized species within the genus Methylosinus was Methylosinus sporium NCIMB 11126^T (96·0 % similarity). Chemotaxonomic and phenotypic data (C_{18 : 1}8 as the major fatty acid, non-motile, no rosette formation) supported the affiliation of strain SV97^T to the genus Methylocystis. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain SV97^T from the two recognized Methylocystis species. Strain SV97^T therefore represents a novel species, for which the name Methylocystis rosea sp. nov. is proposed, with the type strain SV97^T (=DSM 17261^T=ATCC BAA-1196^T).

Published online ahead of print on 28 October 2005 as DOI 10.1099/ijs.0.63912-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, pmoA and nifH gene sequences of Methylocystis rosea SV97^T are AJ414656, AJ414657 and DQ010107, respectively.

A chart showing the growth characteristics of Methylocystis rosea SV97^T at different temperatures and a detailed description of the methods used for fatty acid analysis are available as supplementary material in IJSEM Online.

Present address: Svanhovd Miljøsenter, N-9925 Svanvik, Norway.

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