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Patulibacter minatonensis gen. nov., sp. nov., a novel actinobacterium isolated using an agar medium supplemented with superoxide dismutase, and proposal of Patulibacteraceae fam. nov.

Yko Takahashi¹, Atsuko Matsumoto², Kurimi Morisaki¹ and Satoshi mura^1,2

¹ Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
² The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan

Correspondence
Yko Takahashi
ytakaha{at}lisci.kitasato-u.ac.jp

A novel Gram-positive bacterial strain, designated KV-614^T, was isolated from a soil sample using an agar medium supplemented with superoxide dismutase. Based on 16S rRNA gene sequence analysis, it was found that the strain represented a novel deep-rooting lineage within the class Actinobacteria and clustered with yet-uncultivated bacteria from terrestrial environments and some unidentified strains isolated by unique methods. The most closely related established genus was Conexibacter (92·4 % sequence similarity to Conexibacter woesei DSM 14684^T). Cells of strain KV-614^T were rod-shaped and motile with long flagella. The strain was catalase-positive, oxidase-negative and grew under aerobic conditions. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine and glutamic acid. The peptidoglycan acyl type was acetyl. The only detected isoprenoid quinone was demethylmenaquinone with seven isoprene units (DMK-7). Mycolic acids were not detected. The predominant cellular fatty acid was 9c-octadecenoic acid (C18 : 19c). Minor components were 12-methyl tetradecanoic acid (anteiso-C15 : 0) and 14-methyl hexadecanoic acid (anteiso-C17 : 0). The DNA G+C content was 72 mol%. On the basis of phenotypic and genotypic characteristics, it is proposed that strain KV-614^T represents a new genus and a novel species, Patulibacter minatonensis gen. nov., sp. nov., in the class Actinobacteria. The type strain is KV-614^T (=NRRL B-24346^T=JCM 12834^T=NBRC 100761^T). The creation of the family Patulibacteraceae fam. nov. is proposed to encompass the genus Patulibacter gen. nov.

Published online ahead of print on 9 December 2005 as DOI 10.1099/ijs.0.63796-0.

The GenBank/EMBL/DDBJ accession number for the partial 16S rRNA gene sequence of strain KV-614^T is AB193261.

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