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Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales

¹ Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117 312 Moscow, Russia
² Institute of Physical Chemistry, Russian Academy of Sciences, Leninskiy prospect 31, 119 991 Moscow, Russia
³ Department of Biology, University of Bergen, PO Box 7800, N-5020 Bergen, Norway

Correspondence
A. I. Slobodkin
aslobodkin{at}hotmail.com

A moderately thermophilic, anaerobic bacterium (strain SB91^T) was isolated from a freshwater hot spring at Barguzin Valley, Buryatiya, Russia. Cells of strain SB91^T were straight to slightly curved rods, 0·5–0·6 µm in diameter and 3·0–7·0 µm in length. Formation of endospores was not observed. The temperature range for growth was 26–62 °C, with an optimum at 50 °C. The pH range for growth was 5·5–9·5, with an optimum at pH 7·5–8·0. The substrates utilized by strain SB91^T in the presence of 9,10-anthraquinone 2,6-disulfonate included peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, alanine plus glycine, alanine plus proline, L-valine and n-propanol. Carbohydrates were not utilized. Strain SB91^T reduced amorphous Fe(III) oxide, Fe(III) citrate, Fe(III) EDTA or Fe(III) nitrilotriacetate with peptone, L-valine or n-propanol as an electron donor. Strain SB91^T reduced 9,10-anthraquinone 2,6-disulfonate, thiosulfate, elemental sulfur, fumarate and selenite. Strain SB91^T survived after exposure to gamma-radiation at a dose of 5·4 kGy. The G+C content of the DNA of strain SB91^T was 33 mol%. Analysis of the 16S rRNA gene sequence revealed that the isolated organism belonged to cluster XII of the clostridia. On the basis of its physiological properties and the results of phylogenetic analyses, it is proposed that strain SB91^T represents the sole species of a novel genus, Tepidimicrobium; the name Tepidimicrobium ferriphilum gen. nov., sp. nov. is proposed, with strain SB91^T (=DSM 16624^T=VKM B-2348^T) as the type strain.

Published online ahead of print on 30 September 2005 as DOI 10.1099/ijs.0.63694-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SB91^T is AY656718.

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