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1 Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium
2 BCCMTM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, Ghent 9000, Belgium
3 Institut Rosell Inc., Research and Development, 6100 Royalmount Ave, Montreal, Quebec, Canada, H4P 2R2
Correspondence
Sabri M. Naser
Sabri.Naser{at}Ugent.be
Strain R0052, isolated from a North American dairy starter culture, was initially identified as Lactobacillus acidophilus based on phenotypic analyses. However, upon sequencing the 16S rRNA gene, it became clear that the isolate was very highly related to Lactobacillus suntoryeus, Lactobacillus helveticus and Lactobacillus gallinarum, as similarities ranging from 99·3 to 99·8 % were observed. As an initial screening test to investigate the relatedness of strain R0052 and reference strains of L. suntoryeus, L. helveticus and L. gallinarum, the partial sequences for the genes encoding the alpha subunit of ATP synthase (atpA), RNA polymerase alpha subunit (rpoA), phenylalanyl-tRNA synthase alpha subunit (pheS), the translational elongation factor Tu (tuf), a surface-layer protein (slp) and the Hsp60 chaperonins (groEL) were determined and they revealed high relatedness between all of the strains. The determination of the 16S–23S rRNA internally transcribed spacer (ITS) sequences revealed 98·3–100 % similarity between L. suntoryeus and L. helveticus strains. SDS-PAGE of whole-cell proteins did not distinguish between these species. Fluorescent amplified fragment length polymorphism (FAFLP) could distinguish between these taxa, but they still constituted a single cluster within the L. acidophilus group. Finally, DNA–DNA hybridization experiments between strain R0052 and the type strains of L. helveticus and L. suntoryeus yielded reassociation values above 70 % and confirmed that these names are synonyms.
Published online ahead of print on 7 October 2005 as DOI 10.1099/ijs.0.64001-0.
The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are: 16S rRNA partial gene sequences for strains LMG 11445 and R0052, DQ123572 and DQ123580; atpA partial gene sequences for strains LMG 6413T, LMG 22464T, R0052, LMG 6904T, LMG 9435T, LMG 11445, LMG 13522 and LMG 22465, AM086429–AM086431, AM157788–AM157792, respectively; pheS partial gene sequences for strains LMG 6413T, LMG 22464T, R0052, LMG 6904T, LMG 9435T, LMG 18225, LMG 11445, LMG 11447, LMG 13522 and LMG 22465, AM086432–AM086434, AM157781–AM157787, respectively; rpoA partial gene sequences for strains LMG 6413T, LMG 22464T, R0052, LMG 9435T, LMG 18225, LMG 6904T, LMG 11445, LMG 11447, LMG 13522 and LMG 22465, AM086435–AM086437, AM157774–AM157780, respectively; tuf partial gene sequences for strains LMG 11445, LMG 22465 and R0052, DQ123571, DQ123578 and DQ123584, respectively; groEL partial gene sequences for strains LMG 11445, LMG 22464T, LMG 22465 and R0052, DQ123573, DQ123576, DQ123579 and DQ123581, respectively; 16S–23S ITS gene sequences for strains LMG 11445, LMG 6413T, LMG 22464T, LMG 22465 and R0052, DQ123570, DQ123574, DQ123575, DQ123577 and DQ123582, respectively; slp partial gene sequence for strain R0052, DQ123583.
Additional neighbour-joining trees based on the sequences of six genes and ITS sequences are available as supplementary figures in IJSEM Online.
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