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1 NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusa-kamatari, Kisarazu-shi, Chiba 292-0818, Japan
2 Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan
3 Coastal Branch, Natural History Museum and Institute, 123 Yoshio, Katsuura, Chiba 299-5242, Japan
Correspondence
Yukiyo Fukunaga
fukunaga-yukiyo{at}nite.go.jp
Two bacterial strains, F423T and F10102, were isolated from two ascidians, Polycitor proliferus and Botryllidae sp., respectively, which were collected from a beach on the Boso peninsula in Japan. Cells of both isolates were motile, rod-shaped and formed star-shaped aggregates in the early stage of exponential growth, but were coccoid in stationary growth phase. The results of 16S rRNA gene sequence analysis, fatty acid analysis, DNA–DNA hybridization experiments and physiological and biochemical tests indicated that the two strains were members of a novel species of the genus Pseudovibrio for which the name Pseudovibrio ascidiaceicola sp. nov. is proposed. The type strain is F423T (=NBRC 100514T=IAM 15084T=DSM 16392T=KCTC 12308T).
Published online ahead of print on 13 October 2005 as DOI 10.1099/ijs.0.63879-0.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Pseudovibrio ascidiaceicola F423T is AB175663.
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