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Int J Syst Evol Microbiol 56 (2006), 2819-2823; DOI  10.1099/ijs.0.64191-0
© 2006 International Union of Microbiological Societies

Methylotenera mobilis gen. nov., sp. nov., an obligately methylamine-utilizing bacterium within the family Methylophilaceae

Marina G. Kalyuzhnaya1, Sarah Bowerman2, Jimmie C. Lara3, Mary E. Lidstrom1,3 and Ludmila Chistoserdova1

1 Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA
2 Department of Biology, University of Washington, Seattle, WA 98195, USA
3 Department of Microbiology, University of Washington, Seattle, WA 98195, USA

Correspondence
Ludmila Chistoserdova
milachis{at}u.washington.edu

A novel obligate methylamine utilizer (strain JLW8T), isolated from Lake Washington sediment, was characterized taxonomically. The isolate was an aerobic, Gram-negative bacterium. Cells were rod-shaped and motile by means of a single flagellum. Reproduction was by binary fission and no resting bodies were formed. Growth was observed within a pH range of 5–8.5, with optimum growth at pH 7.5. It utilized methylamine as a single source of energy, carbon and nitrogen. Methylamine was oxidized via methylamine dehydrogenase and formaldehyde was assimilated via the ribulose monophosphate cycle. The cellular fatty acid profile was dominated by C16 : 0{omega}7c and C16 : 0 and the major phospholipid was phosphatidylethanolamine. The DNA G+C content was 54 mol%. 16S rRNA gene sequence analysis indicated that the new isolate was closely related (97–98 % similarity) to a broad group of sequences from uncultured or uncharacterized Betaproteobacteria, but only distantly related (93–96 % similarity) to known methylotrophs of the family Methylophilaceae. Strain JLW8T (=ATCC BAA-1282T=DSM 17540T) is proposed as the type strain of a novel species in a new genus within the family Methylophilaceae, Methylotenera mobilis gen. nov., sp. nov.

Abbreviations: RuMP, ribulose monophosphate

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JLW8T is DQ287786.






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Copyright © 2006 by the International Union of Microbiological Societies.