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Natrinema ejinorense sp. nov., isolated from a saline lake in Inner Mongolia, China

¹ Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
² Noda Institute for Scientific Research, 399 Noda, Noda-shi, Chiba-ken 278-0037, Japan
³ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China
⁴ Department of Biotechnology, University of the Western Cape, Bellville 7535, Cape Town, South Africa
⁵ Genencor International BV, Archimedesweg 30, 2333 CN Leiden, The Netherlands
⁶ Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK

Correspondence
A. Ventosa
ventosa{at}us.es

A Gram-negative, non-motile, neutrophilic, pleomorphic and extremely halophilic archaeon, strain EJ-57^T, was isolated from saline Lake Ejinor in Inner Mongolia, China. Strain EJ-57^T was able to grow at 25–50 °C, required at least 1.8 M NaCl for growth (optimum at 3.4 M NaCl) and grew over a pH range from 6.0 to 8.5 (optimum at pH 7.0). Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. Analysis of the almost complete 16S rRNA gene sequence indicated that the isolate represented a member of the genus Natrinema in the family Halobacteriaceae. Strain EJ-57^T was most closely related to Natrinema versiforme JCM 10478^T (96.2 % sequence similarity), Natrinema pallidum NCIMB 777^T (95.9 % sequence similarity), Natrinema altunense JCM 12890^T (95.8 % sequence similarity) and Natrinema pellirubrum NCIMB 786^T (95.5 % sequence similarity). However, DNA–DNA hybridization experiments showed that strain EJ-57^T was not related to these species, with levels of DNA–DNA relatedness equal to or below 39 %. The major polar lipids of the isolate were C₂₀C₂₀ and C₂₀C₂₅ derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and the disulfated glycolipid S2-DGA-1. The G+C content of the genomic DNA was 64.7 mol%. Comparative analysis of phenotypic characteristics between strain EJ-57^T and recognized Natrinema species supported the conclusion that EJ-57^T represents a novel species within this genus, for which the name Natrinema ejinorense sp. nov. is proposed. The type strain is EJ-57^T (=CECT 7144^T=JCM 13890^T=CGMCC 1.6202^T).

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