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Ferrimonas futtsuensis sp. nov. and Ferrimonas kyonanensis sp. nov., selenate-reducing bacteria belonging to the Gammaproteobacteria isolated from Tokyo Bay

Tatsunori Nakagawa, Takao Iino, Ken-ichiro Suzuki and Shigeaki Harayama

NITE Biological Resource Center, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan

Correspondence
Tatsunori Nakagawa
nakatats{at}brs.nihon-u.ac.jp

Two novel mesophilic, facultatively anaerobic, selenate-reducing bacteria, designated strains FUT3661^T and Asr22-7^T, were isolated from a sediment sample and the alimentary tract of littleneck clams, respectively. Both sources of the samples were collected from the coast of Tokyo Bay, Japan. Cells were Gram-negative rods and motile by means of a polar flagellum. The strains reduced selenate to elemental selenium (Se⁰) and also reduced iron(III) oxyhydroxide, iron(III) citrate, arsenate, manganese(IV) oxide, elemental sulfur and oxygen and used lactate, pyruvate, yeast extract, tryptone and Casamino acids as electron donors and carbon sources. The strains contained both menaquinone (MK-7) and ubiquinones (Q-7 and Q-8) as isoprenoid quinones. The major fatty acids were C_{16 : 0} and C_{16 : 1}9c. The G+C content of the genomic DNA was 58.1 mol% for strain FUT3661^T and 57.2 mol% for strain Asr22-7^T. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were related to members of the genus Ferrimonas (<94.0 % similarities), although the two novel strains formed a separate lineage. 16S rRNA gene sequence similarity between strains FUT3661^T and Asr22-7^T was 96 %. On the basis of this polyphasic analysis, it was concluded that strains FUT3661^T and Asr22-7^T represent two novel species within the genus Ferrimonas, for which the names Ferrimonas futtsuensis sp. nov. (type strain FUT3661^T=NBRC 101558^T=DSM 18154^T) and Ferrimonas kyonanensis sp. nov. (type strain Asr22-7^T=NBRC 101286^T=DSM 18153^T) are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Ferrimonas futtsuensis FUT3661^T and Ferrimonas kyonanensis Asr22-7^T are AB245515 and AB245514, respectively.

Transmission electron micrographs of cells of strains FUT3661^T and Asr22-7^T, figures showing the effects of temperature, NaCl concentration and selenate concentration on growth of these two strains and a table detailing their fatty acid contents are available as supplementary material in IJSEM Online.

Present address: Department of Agricultural and Biological Chemistry, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.

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