HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Material Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hatayama, K. Articles by Nakamura, A. Search for Related Content PubMed PubMed Citation Articles by Hatayama, K. Articles by Nakamura, A. Agricola Articles by Hatayama, K. Articles by Nakamura, A.

Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov.

¹ Division of Integrative Environmental Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1–1–1, Tsukuba, Ibaraki 305–8572, Japan
² Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1–1–1, Bunkyo-ku, Tokyo 113–8657, Japan
³ Institute of Hyperthermophiles, Motobu-Noge Hospital, Aza-Ohama 880–1, Motobu, Okinawa 905–0212, Japan

Correspondence
Akira Nakamura
a-nak{at}agbi.tsukuba.ac.jp

Two thermophilic strains, designated 607^T and 606b, were isolated from a compost pile in Japan. The novel strains were Gram-positive, aerobic, spore-forming rods. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains 607^T and 606b were closely related to Bacillus naganoensis (94.0–94.1 % similarity) and separated from clusters of the related genera Bacillus (<91.9 %) and Sporolactobacillus (91.0–92.5 %). In addition, some chemotaxonomic and physiological characteristics of strains 607^T and 606b differed from those of B. naganoensis and the two related genera. Several differences in physiological characteristics and 16S–23S rRNA gene internal transcribed spacer region nucleotide sequences were observed between strains 607^T and 606b; however, DNA–DNA hybridization indicated that these two strains belonged to the same species. From these results, it is proposed that strains 607^T and 606b represent the type species of a new genus, Tuberibacillus calidus gen. nov., sp. nov., with strain 607^T (=JCM 13397^T=DSM 17572^T) as the type strain. In addition, the results of phylogenetic analyses, as well as chemotaxonomic and physiological characterization, indicated that B. naganoensis and Bacillus laevolacticus did not belong to the genus Bacillus. Based on these results, it is proposed that B. naganoensis and B. laevolacticus should be transferred to Pullulanibacillus naganoensis gen. nov., comb. nov. and Sporolactobacillus laevolacticus comb. nov., respectively.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Tuberibacillus calidus 607^T and 606b are AB231786 and AB231785, respectively. The accession numbers for the nucleotide sequences of the 16S–23S ITS regions of the Tuberibacillus, Pullulanibacillus and Sporolactobacillus strains determined in this work are AB231787–AB231797.

An additional phylogenetic tree, phase-contrast microscope images of cells of Tuberibacillus calidus sp. nov. and a table detailing major cellular fatty acids for the novel isolates and related recognized species are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				Y.-H. Chang, M. Y. Jung, I.-S. Park, and H.-M. Oh Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil Int J Syst Evol Microbiol, October 1, 2008; 58(10): 2316 - 2320. [Abstract] [Full Text] [PDF]

				J.-M. Lim, C. O. Jeon, J. R. Lee, D.-J. Park, and C.-J. Kim Bacillus kribbensis sp. nov., isolated from a soil sample in Jeju, Korea Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2912 - 2916. [Abstract] [Full Text] [PDF]