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Reclassification of Lactobacillus amylophilus LMG 11400 and NRRL B-4435 as Lactobacillus amylotrophicus sp. nov.

Sabri M. Naser^1,2,, Marc Vancanneyt², Cindy Snauwaert², Gino Vrancken³, Bart Hoste², Luc De Vuyst³ and Jean Swings^1,2

¹ Laboratory of Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium
² BCCM^TM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium
³ Research Group of Industrial Microbiology and Food Biotechnology, Department of Applied Biological Sciences and Engineering, Vrije Universiteit Brussel (VUB), Pleinlaan 2, B-1050 Brussels, Belgium

Correspondence
Sabri M. Naser
Sabri.Naser{at}najah.edu

The taxonomic position of six Lactobacillus amylophilus strains isolated from swine waste-corn fermentations was reinvestigated. All strains were included in a multilocus sequence analysis (MLSA) study for species identification of Lactobacillus using the genes encoding the phenylalanyl-tRNA synthase alpha subunit (pheS) and RNA polymerase alpha subunit (rpoA). Partial pheS and rpoA gene sequences showed that strains LMG 11400 and NRRL B-4435 represent a separate lineage that is distantly related to the type strain of L. amylophilus, LMG 6900^T, and to three other strains of the species. The MLSA data showed that the two strains LMG 11400 and NRRL B-4435 constituted a distinct cluster, sharing 100 % pheS and rpoA gene sequence similarity. The other reference strains clustered together with the type strain of L. amylophilus, LMG 6900^T, and were clearly differentiated from strains LMG 11400 and NRRL B-4435 (80 and 89 % pheS and rpoA gene sequence similarity, respectively). The 16S rRNA gene sequences of the latter two strains are 100 % identical, with the nearest phylogenetic neighbour L. amylophilus LMG 6900^T showing only 97.2 % 16S rRNA gene sequence similarity. Further polyphasic taxonomic study based on whole-cell protein fingerprinting, DNA–DNA hybridization and biochemical features demonstrated that the two strains represent a single, novel Lactobacillus species, for which the name Lactobacillus amylotrophicus sp. nov. is proposed. The type strain is LMG 11400^T (=NRRL B-4436^T=DSM 20534^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of LMG 11400^T and NRRL B-4435 are AM236149 and AM236150, respectively. Other accession numbers reported in this paper are: partial pheS gene sequences for strains LMG 11400^T, NRRL B-4435, NRRL B-4438, NRRL B-4439 and NRRL B-4440, AM236139–AM236143; partial rpoA gene sequences for strains LMG 11400^T, NRRL B-4440, NRRL B-4435, NRRL B-4438 and NRRL B-4439, AM236144–AM236148.

A distance-matrix tree based on 16S rRNA gene sequences is available as supplementary material in IJSEM Online.

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