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Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates

Marina G. Kalyuzhnaya^1,, Paolo De Marco^2,, Sarah Bowerman³, Catarina C. Pacheco², Jimmie C. Lara⁴, Mary E. Lidstrom^1,4 and Ludmila Chistoserdova¹

¹ Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA
² Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Campo Alegre, 823, 4150-180 Porto, Portugal
³ Department of Biology, University of Washington, Seattle, WA 98195, USA
⁴ Department of Microbiology, University of Washington, Seattle, WA 98195, USA

Correspondence
Ludmila Chistoserdova
milachis{at}u.washington.edu

The taxonomic positions and phylogenetic relationships of two new methylotrophic isolates from Lake Washington (USA) sediment, FAM5^T and 500, and the previously described methylotrophic strain EHg5 isolated from contaminated soil in Estarreja (Portugal) were investigated. All three strains were facultative methylotrophs capable of growth on a variety of C₁ and multicarbon compounds. Optimal growth occurred at pH 7.5–8 and 30–37 °C. The major fatty acids were C_{16 : 1}7c and C_{16 : 0}. The major quinone was ubiquinone Q8. Neither methanol dehydrogenase nor methanol oxidase activities were detectable in cells grown on methanol, suggesting an alternative, as-yet unknown, mechanism for methanol oxidation. The isolates assimilated C₁ units at the level of formaldehyde, via the serine cycle. The DNA G+C content of the strains ranged between 64 and 65 mol%. 16S rRNA gene sequence similarity between the three new isolates was 99.85–100 %, but was below 94 % with other members of the Betaproteobacteria, indicating that the isolates represent a novel taxon. Based on physiological, phenotypic and genomic characteristics of the three isolates, a new genus, Methyloversatilis gen. nov., is proposed within the family Rhodocyclaceae. The type strain of Methyloversatilis universalis gen. nov., sp. nov. is FAM5^T (=CCUG 52030^T=JCM 13912^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, fae and fhcD gene sequences of strain FAM5^T are DQ442273, DQ457109 and DQ457110, and that for the 16S rRNA gene sequence of strain 500 is DQ923115.

These authors contributed equally to this work.

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