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Int J Syst Evol Microbiol 56 (2006), 2473-2479; DOI  10.1099/ijs.0.64357-0
© 2006 International Union of Microbiological Societies

Morganella psychrotolerans sp. nov., a histamine-producing bacterium isolated from various seafoods

Jette Emborg1, Paw Dalgaard1 and Peter Ahrens2

1 Danish Institute for Fisheries Research, Department of Seafood Research, c/o Technical University of Denmark, Søltofts Plads, Building 221, DK-2800 Kgs. Lyngby, Denmark
2 Danish Institute for Food and Veterinary Research, Bülowsvej 27, DK-1790 Copenhagen V, Denmark

Correspondence
Jette Emborg
jem{at}difres.dk

Mesophilic Morganella morganii (n=6) and psychrotolerant M. morganii-like isolates from various seafoods (n=13), as well as clinical M. morganii isolates (n=3), were characterized by using a polyphasic approach including multi-locus sequencing. Based on the phylogenetic analysis, the 22 strains were divided into two distinct groups comprising mesophilic and psychrotolerant isolates, respectively. This classification was supported by DNA–DNA hybridization studies, whereby a psychrotolerant isolate (strain U2/3T) showed 41.0 and 17.8 % relatedness to the type strains of the mesophilic species Morganella morganii subsp. morganii (strain LMG 7874T) and Morganella morganii subsp. sibonii (strain DSM 14850T), respectively. Analysis of the 16S rRNA gene sequences showed a similarity of 98.6 % between mesophilic and psychrotolerant isolates. However, fragments of seven protein-encoding housekeeping genes (atpD, dnaN, gyrB, hdc, infB, rpoB and tuf) all showed less than 90.9 % sequence similarity between the two groups. The psychrotolerant isolates grew at 0–2 °C and also differed from the mesophilic M. morganii isolates with respect to growth at 37 °C and in 8.5 % (w/v) NaCl and fermentation of D-galactose. The psychrotolerant strains appear to represent a novel species, for which the name Morganella psychrotolerans sp. nov. is proposed. The type strain is U2/3T (=LMG 23374T=DSM 17886T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, atpD, dnaN, gyrB, hdc, infB, rpoB and tuf gene fragments determined in this study are given in Fig. 1 and Supplementary Figs S1–S7.

Neighbour-joining trees based on atpD, dnaN, gyrB, hdc, infB, rpoB and tuf genes and tables detailing primer sequences and annealing temperatures used in PCR and whole-cell fatty acid content and characteristics of isolates of Morganella psychrotolerans sp. nov. and Morganella morganii are available as supplementary material in IJSEM Online.






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Copyright © 2006 by the International Union of Microbiological Societies.