HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures and Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jean, W. D. Articles by Shieh, W. Y. Search for Related Content PubMed PubMed Citation Articles by Jean, W. D. Articles by Shieh, W. Y. Agricola Articles by Jean, W. D. Articles by Shieh, W. Y.

Bowmanella denitrificans gen. nov., sp. nov., a denitrifying bacterium isolated from seawater from An-Ping Harbour, Taiwan

¹ Center for General Education, Leader University, No. 188, Sec. 5, An-Chung Road, Tainan, Taiwan
² School of Medicine, China Medical University, No. 91, Shyue-Shyh Road, Taichung, Taiwan
³ Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan

Correspondence
Wung Yang Shieh
winyang{at}ntu.edu.tw

A heterotrophic, non-fermentative, denitrifying isolate, designated strain BD1^T, was obtained from a seawater sample collected in the shallow coastal region of An-Ping Harbour, Tainan, Taiwan. The cells of strain BD1^T were Gram-negative. Cells grown in broth cultures were curved rods that were motile by means of a single polar flagellum. Growth occurred between 10 and 40 °C, with an optimum at 30–35 °C. Strain BD1^T grew in NaCl levels of 0–10 %, with better growth occurring at 1–3 %. It grew aerobically and could achieve anaerobic growth by adopting a denitrifying metabolism with nitrate or nitrous oxide as the terminal electron acceptor. The major fatty acids were C_{16 : 0}, C_{18 : 1}7c and summed feature 3 (C_{16 : 1}7c and/or C_{15 : 0} iso 2-OH). The polar lipids consisted of phosphatidylethanolamine (56.6 %) and phosphatidylglycerol (43.4 %). The isoprenoid quinones were Q-8 (81.5 %), Q-9 (11.1 %) and Q-10 (7.4 %). The DNA G+C content was 50.0 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD1^T formed a distinct lineage in the Gammaproteobacteria and that it exhibited the highest level of sequence similarity with species of the genera Alteromonas (92.8–93.7 %), Aestuariibacter (93.0 %), Glaciecola (90.4–92.7 %) and Salinimonas (91.8 %). Strain BD1^T was distinguishable from species of these genera by the presence of Q-9 and Q-10. Phenotypically, strain BD1^T was also distinguishable from species of these genera in that it did not require NaCl for growth and was capable of denitrification. On the basis of the polyphasic data from this study, the isolate represents a novel species within a novel genus, for which the name Bowmanella denitrificans gen. nov., sp. nov. is proposed. The type strain of Bowmanella denitrificans is BD1^T (=BCRC 17491^T=JCM 13378^T).

Fatty acid compositions of strain BD1^T and related strains, electron micrographs and growth curves are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				Q. Lai, J. Yuan, B. Wang, F. Sun, N. Qiao, T. Zheng, and Z. Shao Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium Int J Syst Evol Microbiol, July 1, 2009; 59(7): 1579 - 1582. [Abstract] [Full Text] [PDF]

				H.-H. Chiu, W. Y. Shieh, S. Y. Lin, C.-M. Tseng, P.-W. Chiang, and I. Wagner-Dobler Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1209 - 1216. [Abstract] [Full Text] [PDF]