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Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil

Angel Valverde^1,, Pedro Delvasto², Alvaro Peix¹, Encarna Velázquez³, Ignacio Santa-Regina¹, Antonio Ballester², Claudino Rodríguez-Barrueco¹, Camino García-Balboa⁴ and José M. Igual¹

¹ Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas, 37071 Salamanca, Spain
² Biohydrometallurgy Research Group, Department of Materials Science and Metallurgical Engineering, Universidad Complutense de Madrid, 28040 Madrid, Spain
³ Departamento de Microbiología y Genética, Universidad de Salamanca, 37007 Salamanca, Spain
⁴ Department of Industrial Technology, Universidad Alfonso X ‘El Sabio’, Av. de la Universidad 1, 28691 Villanueva de la Cañada, Madrid, Spain

Correspondence
José M. Igual
igual{at}usal.es

A Gram-negative, non-spore-forming bacterial strain with the ability to solubilize highly insoluble phosphatic minerals was isolated from a high-phosphorous iron ore from Minas Gerais State, Brazil. This strain, designated FeGl01^T, was subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis indicated that it formed a distinct phylogenetic lineage within the genus Burkholderia together with several other species of the genus, e.g. Burkholderia sacchari, Burkholderia tropica and Burkholderia unamae. Partial nucleotide sequencing and analysis of the recA gene roughly corroborated the phylogenetic position of strain FeGl01^T within the genus Burkholderia. The chemotaxonomic properties of strain FeGl01^T, such as ubiquinone Q-8 as the predominant quinone system and C_{16 : 0}, C_{17 : 0} cyclo, C_{18 : 1}7c and C_{19 : 0}8c cyclo as the major fatty acids, were also consistent with its classification within the genus Burkholderia. DNA–DNA hybridization experiments between strain FeGl01^T and the type strains of B. unamae, B. sacchari and B. tropica yielded reassociation values of 40 % or lower, which, together with qualitative and quantitative differences in fatty acid composition and with differences in several phenotypic traits, support the separation of the new isolate from the phylogenetically most closely related species. Therefore, it is suggested that strain FeGl01^T represents a novel species of the genus Burkholderia, for which the name Burkholderia ferrariae sp. nov. is proposed. The type strain is FeGl01^T (=LMG 23612^T=CECT 7171^T=DSM 18251^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of B. ferrariae FeGl01^T is DQ514537 and the accession numbers for the recA gene sequences of B. ferrariae FeGl01^T, B. unamae MTI-641^T and B. tropica Ppe8^T are DQ514538–DQ514540, respectively.

An extended neighbour-joining tree showing the phylogenetic position of strain FeGl01^T based on its 16S rRNA gene sequence is available as supplementary material in IJSEM Online.

Present address: Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

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