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Description of Wautersiella falsenii gen. nov., sp. nov., to accommodate clinical isolates phenotypically resembling members of the genera Chryseobacterium and Empedobacter

¹ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Germany
² Microbiology Unit, Faculty of Medicine, University of Louvain, Brussels, Belgium
³ Department of Clinical Chemistry, Microbiology and Immunology, University of Ghent, Ghent, Belgium

Correspondence
Mario Vaneechoutte
Mario.Vaneechoutte{at}UGent.be

A total of 26 isolates of non-fermenting, Gram-negative rods, obtained between 1980 and 2004 by various clinical laboratories in Belgium, with phenotypic characteristics resembling those of members of the genera Chryseobacterium and Empedobacter (indole-positive) and a biochemical profile resembling that of CDC group II-h, but urease-positive, were collected at the Université Catholique de Louvain Microbiology Laboratory, Belgium. The 16S rRNA gene sequences were determined for most of the isolates and showed 94–95 % similarity with the type strain of Empedobacter brevis as the closest relative, indicating that these isolates might belong to a separate genus. Furthermore, the 16S rRNA gene sequences of the isolates were similar, but two clusters (genomovars) could be distinguished. The sequence similarities were 99.5–100 % for the 14 isolates of genomovar 1 and 99.4–100 % for the 12 isolates of genomovar 2. The similarity between the two clusters was 98.3–99.5 %. The presence of two clearly different groups was corroborated by using tRNA intergenic length polymorphism analysis, which also enabled differentiation of the novel species from all other species studied thus far using this technique. DNA–DNA hybridization results excluded a close relatedness to Empedobacter brevis. The DNA G+C contents of the reference strains of genomovars 1 and 2 were 33.8±0.4 and 34.4±0.2 mol%, respectively. The name Wautersiella falsenii gen. nov., sp. nov., is proposed for this group, comprising two closely related genomovars. The type strain of the species and reference strain for genomovar 1 is NF 993^T (=CCUG 51536^T=CIP 108861^T), which was isolated from a surgical wound. The reference strain for genomovar 2 is NF 770 (=CCUG 51537=CIP 108860), which was isolated from blood.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Wautersiella falsenii genomovar 1 strains NF 993^T, NF 1182, NF 880, NF 777, NF 869, NF 289, NF 1240, NF 1241, NF 835, NF 1242, NF 1243 and NF 1244 are AM084341 and AM238680–AM238690, respectively, those of Wautersiella falsenii genomovar 2 strains NF 770 (reference strain), NF 622, NF 1159, NF 1249, NF 1140, NF 1084, NF 1080, NF 1136, NF 203, NF 316 and NF 58 are AM084342 and AM238670–AM238679, respectively, and those of Empedobacter brevis strain LMG 4011^T and group CDC II-h strain NF 802 are AM177497 and AM261868, respectively.

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				M. Vaneechoutte, P. Kampfer, T. De Baere, V. Avesani, M. Janssens, and G. Wauters Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2623 - 2628. [Abstract] [Full Text] [PDF]