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Isolates of ‘Candidatus Nostocoida limicola’ Blackall et al. 2000 should be described as three novel species of the genus Tetrasphaera, as Tetrasphaera jenkinsii sp. nov., Tetrasphaera vanveenii sp. nov. and Tetrasphaera veronensis sp. nov.

C. M. McKenzie¹, E. M. Seviour¹, P. Schumann², A. M. Maszenan^1,, J.-R. Liu^1,, R. I. Webb³, P. Monis⁴, C. P. Saint⁴, U. Steiner² and R. J. Seviour¹

¹ Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3552, Australia
² DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany
³ Centre for Microscopy and Microanalysis, Department of Microbiology, The University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia
⁴ Australian Water Quality Centre, Bolivar, South Australia 5108, Australia

Correspondence
R. J. Seviour
r.seviour{at}latrobe.edu.au

Despite differences in their morphologies, comparative analyses of 16S rRNA gene sequences revealed high levels of similarity (>94 %) between strains of the filamentous bacterium ‘Candidatus Nostocoida limicola’ and the cocci Tetrasphaera australiensis and Tetrasphaera japonica and the rod Tetrasphaera elongata, all isolated from activated sludge. These sequence data and their chemotaxonomic characters, including cell wall, menaquinone and lipid compositions and fingerprints of their 16S–23S rRNA intergenic regions, support the proposition that these isolates should be combined into a single genus containing six species, in the family Intrasporangiaceae in the Actinobacteria. This suggestion receives additional support from DNA–DNA hybridization data and when partial sequences of the rpoC1 gene are compared between these strains. Even though few phenotypic characterization data were obtained for these slowly growing isolates, it is proposed, on the basis of the extensive chemotaxonomic and molecular evidence presented here, that ‘Candidatus N. limicola’ strains Ben 17, Ben 18, Ben 67, Ben 68 and Ben 74 all be placed into the species Tetrasphaera jenkinsii sp. nov. (type strain Ben 74^T=DSM 17519^T=NCIMB 14128^T), ‘Candidatus N. limicola’ strain Ben 70 into Tetrasphaera vanveenii sp. nov. (type strain Ben 70^T=DSM 17518^T=NCIMB 14127^T) and ‘Candidatus N. limicola’ strains Ver 1 and Ver 2 into Tetrasphaera veronensis sp. nov. (type strain Ver 1^T=DSM 17520^T=NCIMB 14129^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains Ben 68, Ben 70 and Ben 74 are DQ007319–DQ007321, respectively, and the accession numbers for the rpoC1 gene sequences determined in this study are DQ007322–DQ007334, as detailed in Fig. 4.

Fatty acid profiles of selected strains of ‘Candidatus N. limicola’ and related strains are available in IJSEM Online.

Present address: Environmental Engineering Research Centre, School of Civil and Environmental Engineering, Nanyang Technological University, Singapore.

Present address: Department of Chemistry and Biology, Ryerson University, Toronto, Ontario M5B 2K3, Canada.

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