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Int J Syst Evol Microbiol 56 (2006), 2253-2263; DOI  10.1099/ijs.0.64417-0
© 2006 International Union of Microbiological Societies

Phylogenetic evaluation of cyanobacteria preserved as historic herbarium exsiccata

Katarzyna A. Palinska1, Christian F. Thomasius1, Jürgen Marquardt1 and Stjepko Golubic2

1 Geomicrobiology, ICBM, Carl von Ossietzky University of Oldenburg, 26111 Oldenburg, Germany
2 Biological Science Center, Boston University, 5 Cummington Street, Boston, MA 02215, USA

Correspondence
Katarzyna A. Palinska
k.palinska{at}uni-oldenburg.de

Dried herbarium specimens of cyanobacteria (exsiccata) deposited over 100 years ago were analysed and characterized using combined morphological and molecular approaches. Six representative coccoid and filamentous cyanobacteria from two historic collections and a 15-year-old air-dried environmental sample were studied. Morphological features observed by light and electron microscopy were correlated with the results of 16S rRNA gene sequencing. Historic identifications achieved by means of classical morphology could thus be confirmed by extracted, amplified and sequenced 16S rRNA gene fragments. The results of this study open the possibility of providing genotypic characterizations to botanical type specimens, thus reconciling the botanical and bacteriological approaches to the taxonomic treatment of these micro-organisms.

Abbreviations: EPS, extracellular polymer substances; LM, light microscope/microscopy; SEM, scanning electron microscope/microscopy; TEM, transmission electron microscope/microscopy

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences determined in this study for the seven cyanobacterial species are shown in Table 1.






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