HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures and Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ghosh, W. Articles by Roy, P. Search for Related Content PubMed PubMed Citation Articles by Ghosh, W. Articles by Roy, P. Agricola Articles by Ghosh, W. Articles by Roy, P.

Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant

Department of Microbiology, Bose Institute, P-1/12, CIT Scheme VII-M, Kolkata – 700 054, India

Correspondence
Wriddhiman Ghosh
Wriman{at}rediffmail.com

The bacterial strain SJT^T, along with 15 other mesophilic, neutrophilic and facultatively sulfur-oxidizing chemolithotrophic isolates, was isolated by enrichment on reduced sulfur compounds as the sole energy and electron source from soils immediately adjacent to the roots of Clitoria ternatea, a slender leguminous herb of the Lower Gangetic plains of India. Strain SJT^T was able to oxidize thiosulfate and elemental sulfur for chemolithoautotrophic growth. 16S rRNA and recA gene sequence-based phylogenetic analyses showed that the Gram-negative rod-shaped bacterium belonged to the genus Mesorhizobium and was most closely related to Mesorhizobium loti, Mesorhizobium plurifarium, Mesorhizobium amorphae and Mesorhizobium chacoense. Unequivocally low 16S rRNA (<97 %) and recA (88 %) gene sequence similarities to all existing species of the most closely related genera, a unique fatty acid profile, a distinct G+C content (59·6 mol%) and phenotypic characteristics all suggested that strain SJT^T represents a novel species. DNA–DNA hybridization and SDS-PAGE analysis of whole-cell proteins also confirmed the taxonomic uniqueness of SJT^T. It is therefore proposed that isolate SJT^T (=LMG 22697^T=MTCC 7001^T) be classified as the type strain of a novel species, Mesorhizobium thiogangeticum sp. nov.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and recA gene sequences of the strain SJT^T are AJ864462 and AM040610, respectively.

Fatty acid profiles, a phenogram derived from UPGMA/S_SM analysis and SDS-PAGE analysis of whole-cell proteins of strain SJT^T and related species are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				F. Q. Wang, E. T. Wang, J. Liu, Q. Chen, X. H. Sui, W. F. Chen, and W. X. Chen Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1192 - 1199. [Abstract] [Full Text] [PDF]

				K. G. Nandasena, G. W. O'Hara, R. P. Tiwari, A. Willlems, and J. G. Howieson Mesorhizobium ciceri biovar biserrulae, a novel biovar nodulating the pasture legume Biserrula pelecinus L. Int J Syst Evol Microbiol, May 1, 2007; 57(5): 1041 - 1045. [Abstract] [Full Text] [PDF]

				W. M. Sattley and M. T. Madigan Isolation, Characterization, and Ecology of Cold-Active, Chemolithotrophic, Sulfur-Oxidizing Bacteria from Perennially Ice-Covered Lake Fryxell, Antarctica Appl. Envir. Microbiol., August 1, 2006; 72(8): 5562 - 5568. [Abstract] [Full Text] [PDF]