HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Maruyama, T. Articles by Kato, K. Search for Related Content PubMed PubMed Citation Articles by Maruyama, T. Articles by Kato, K. Agricola Articles by Maruyama, T. Articles by Kato, K.

Sphingosinicella microcystinivorans gen. nov., sp. nov., a microcystin-degrading bacterium

¹ Marine Works Japan Ltd, Kamariyahigashi, 2-16-32 4F, Kanazawa-ku, Yokohama 236-0042, Japan
² Department of Environmental Sciences, Faculty of Science, Shinshu University, Asahi, 3-1-1, Matsumoto 390-8621, Japan
³ Department of Microbiology and Immunology, Graduate School of Medicine, Tohoku University, Seiryo-machi, 2-1, Aoba-ku, Sendai 980-8575, Japan
⁴ Hitachi Plant Engineering & Construction Co., Ltd, Matsudo 271-0064, Japan
⁵ Department of Laboratory Sciences, School of Health Sciences, Faculty of Medicine, Gunma University, Showa-machi 3chome, 39-15, Maebashi 371-8514, Japan
⁶ Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi 441-8580, Japan
⁷ Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Oya, 836, Shizuoka 422-8529, Japan

Correspondence
Tomoko Maruyama
maruyamat{at}mwj.co.jp

Three strains of bacteria that degrade the cyanobacterial hepatotoxin microcystin, Y2^T, MDB2 and MDB3, were isolated from a eutrophic lake, Lake Suwa, and the Tenryu River, Japan, and characterized. These strains were aerobic and chemo-organotrophic and their cells were Gram-negative, non-spore-forming rods, motile by means of single polar flagella. Yellow-pigmented colonies were formed on nutrient agar media. The strains assimilated only citrate among the organic compounds tested as carbon sources. The G+C content of genomic DNA ranged from 63·6 to 63·7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolates formed a tight cluster within the family Sphingomonadaceae but were clearly separate from established genera of this family, e.g. Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis; sequence similarities between the new isolates and type strains from established genera ranged from 90·9 to 94·9 %. Chemotaxonomic and phenotypic data supported the conclusion that these strains were members of the family Sphingomonadaceae. The major components of the cellular fatty acids were 18 : 17c (36–41 %) and 16 : 17c (33–36 %). Hydroxy fatty acids were mainly 2-OH 14 : 0 (11–13 %), and 3-OH fatty acids were absent. Glycosphingolipids were detected. Ubiquinone-10 and homospermidine were present as the major quinine and polyamine, respectively. Thus, it is proposed that the three strains represent a new genus and species of the family Sphingomonadaceae with the name Sphingosinicella microcystinivorans gen. nov., sp. nov. The type strain is Y2^T (=KCTC 12019^T=JCM 13185^T).

The GenBank/EMBL/DDBJ/accession numbers for the 16S rRNA gene sequences of strains Y2^T, MDB2 and MDB3 are AB084247, AB219940 and AB219941.

This article has been cited by other articles:

				J.-H. Yoon, S.-J. Kang, J.-S. Lee, S.-W. Nam, W. Kim, and T.-K. Oh Sphingosinicella soli sp. nov., isolated from an alkaline soil in Korea Int J Syst Evol Microbiol, January 1, 2008; 58(1): 173 - 177. [Abstract] [Full Text] [PDF]

				R.-M. Wittich, H.-J. Busse, P. Kampfer, A. J. Macedo, M. Tiirola, M. Wieser, and W.-R. Abraham Sphingomonas fennica sp. nov. and Sphingomonas haloaromaticamans sp. nov., outliers of the genus Sphingomonas Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1740 - 1746. [Abstract] [Full Text] [PDF]

				M. K. Kim, K. Schubert, W.-T. Im, K.-H. Kim, S.-T. Lee, and J. Overmann Sphingomonas kaistensis sp. nov., a novel alphaproteobacterium containing pufLM genes Int J Syst Evol Microbiol, July 1, 2007; 57(7): 1527 - 1534. [Abstract] [Full Text] [PDF]

				G. S. N. Reddy and F. Garcia-Pichel Sphingomonas mucosissima sp. nov. and Sphingomonas desiccabilis sp. nov., from biological soil crusts in the Colorado Plateau, USA Int J Syst Evol Microbiol, May 1, 2007; 57(5): 1028 - 1034. [Abstract] [Full Text] [PDF]

				R.-M. Wittich, H.-J. Busse, P. Kampfer, M. Tiirola, M. Wieser, A. J. Macedo, and W.-R. Abraham Sphingobium aromaticiconvertens sp. nov., a xenobiotic-compound-degrading bacterium from polluted river sediment Int J Syst Evol Microbiol, February 1, 2007; 57(2): 306 - 310. [Abstract] [Full Text] [PDF]

				B. Geueke, H.-J. Busse, T. Fleischmann, P. Kampfer, and H.-P. E. Kohler Description of Sphingosinicella xenopeptidilytica sp. nov., a beta-peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans Int J Syst Evol Microbiol, January 1, 2007; 57(1): 107 - 113. [Abstract] [Full Text] [PDF]

				O. Prakash and R. Lal Description of Sphingobium fuliginis sp. nov., a phenanthrene-degrading bacterium from a fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov. Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2147 - 2152. [Abstract] [Full Text] [PDF]