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1 Centro de Biologia da Universidade do Minho (CBUM), Departamento de Biologia, Campus de Gualtar, 4710-057 Braga, Portugal
2 National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
Correspondence
Célia Pais
cpais{at}bio.uminho.pt
Two yeast strains, 153MT and NCYC 3133, isolated from clinical sources in separate hospitals were found to be almost identical in the sequences of the D1/D2 domain of large-subunit rDNA, the PCR fingerprinting profiles and physiological characteristics. The isolates are phenotypically similar, although not identical, to Candida glabrata and Kluyveromyces delphensis (recently renamed Nakaseomyces delphensis). Sequence analysis of the 26S rDNA D1/D2 gene variable region revealed that the two clinical isolates were closely related phylogenetically to C. glabrata and K. delphensis, but differed sufficiently to justify their assignment as representatives of a separate species. The name Candida bracarensis sp. nov. is proposed for the novel species with the type strain 153MT (=CBS 10154T=NCYC D3853T=CECT 12000T).
Published online ahead of print on 13 October 2005 as DOI 10.1099/ijs.0.64076-0.
The GenBank/EMBL/DDBJ accession number for the 26S rDNA D1/D2 domain and complete ITS and 5·8S rDNA gene sequences of Candida bracarensis 153MT are AY589572 and AY589573, respectively.
A figure showing the pairwise alignment of the 26S rDNA D1/D2 sequences of C. glabrata, K. delphensis and strain 153MT is available as supplementary material in IJSEM Online.
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