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Int J Syst Evol Microbiol 56 (2006), 289-293; DOI  10.1099/ijs.0.63827-0
© 2006 International Union of Microbiological Societies

Pseudozyma hubeiensis sp. nov. and Pseudozyma shanxiensis sp. nov., novel ustilaginomycetous anamorphic yeast species from plant leaves

Qi-Ming Wang, Jian-Hua Jia and Feng-Yan Bai

Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China

Correspondence
Feng-Yan Bai
baify{at}im.ac.cn

Among basidiomycetous yeast strains isolated from wilting leaves of various plants in China, two groups of Pseudozyma strains were distinguished from the others by morphological and physiological characterization. Molecular taxonomic analysis based on sequencing of the large subunit (26S) rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region confirmed that the two groups represent two novel species. They are proposed as Pseudozyma hubeiensis sp. nov. (type strain WS 6.4T=AS 2.2493T=CBS 10077T) and Pseudozyma shanxiensis sp. nov. (type strain SH 64T=AS 2.2523T=CBS 10075T). The phylogenetic relationships of the novel species with described Pseudozyma species and related ustilagomycetous teleomorphs were analysed based on the combined sequences of ITS and D1/D2. The phenotypic diagnosis of Pseudozyma was emended because of the negative inositol assimilation reaction of P. hubeiensis sp. nov., which was closely related to the type species of the genus, Pseudozyma prolifica.


Abbreviations: ITS, internal transcribed spacer

The GenBank/EMBL/DDBJ accession numbers for the ITS region sequences of strains WS 6.4T and SH 64T are DQ008954 and DQ008956 and those for the 26S rRNA gene D1/D2 domain sequences are DQ008953 and DQ008955, respectively.







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