| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology Department, Portland State University, PO Box 751, Portland, OR 97207-0751, USA
Correspondence
Melissa M. Kendall
kendallm{at}pdx.edu
Three novel strains of methylotrophic methanogens were isolated from Skan Bay, Alaska, by using anaerobic cultivation techniques. The water was 65 m deep at the sampling site. Strains AK-4 (=OCM 774), AK-5T (=OCM 775T=DSM 17273T) and AK-9 (=OCM 793) were isolated from the sulfate-reducing zone of the sediments. Each of the strains was a non-motile coccus and occurred singly. Cells grew with trimethylamine as a catabolic substrate and strain AK-4 could also catabolize methanol. Yeast extract and trypticase peptones were not required for growth, but their addition to the culture medium slightly stimulated growth. Each of the strains grew at temperatures of 5–28 °C; they were slight halophiles and grew fastest in the neutral pH range. Analysis of the 16S rRNA gene sequences indicated that strain AK-4 was most closely related to Methanosarcina baltica. DNA–DNA hybridization studies showed 88 % relatedness, suggesting that strain AK-4 represents a novel strain within this species. Strains AK-5T and AK-9 had identical 16S rRNA gene sequences that were most closely related to the sequence of Methanococcoides burtonii (99·8 % sequence similarity). DNA–DNA hybridization studies showed that strains AK-5T and AK-9 are members of the same species (88 % relatedness value), but strain AK-5T had a DNA–DNA relatedness value of only 55 % to Methanococcoides burtonii. This indicates that strains AK-5T and AK-9 should be considered as members of a novel species in the genus Methanococcoides. We propose the name Methanococcoides alaskense sp. nov., with strain AK-5T (=OCM 775T=DSM 17273T) as the type strain.
Published online ahead of print on 12 August 2005 as DOI 10.1099/ijs.0.63886-0.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains AK-5T, AK-9 and AK-4 are AY941801, AY941802 and AY663809, respectively.
Graphs showing the effect of environmental factors on the growth rates of strains AK-4, AK-5T and AK-9 are available as supplementary material in IJSEM Online.
This article has been cited by other articles:
|
|
 |
|
  S. Sakai, H. Imachi, S. Hanada, A. Ohashi, H. Harada, and Y. Kamagata Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage 'Rice Cluster I', and proposal of the new archaeal order Methanocellales ord. nov. Int J Syst Evol Microbiol, April 1, 2008; 58(4): 929 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. N. Perreault, D. T. Andersen, W. H. Pollard, C. W. Greer, and L. G. Whyte Characterization of the Prokaryotic Diversity in Cold Saline Perennial Springs of the Canadian High Arctic Appl. Envir. Microbiol., March 1, 2007; 73(5): 1532 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Kendall, G. D. Wardlaw, C. F. Tang, A. S. Bonin, Y. Liu, and D. L. Valentine Diversity of Archaea in Marine Sediments from Skan Bay, Alaska, Including Cultivated Methanogens, and Description of Methanogenium boonei sp. nov. Appl. Envir. Microbiol., January 1, 2007; 73(2): 407 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Kendall, Y. Liu, M. Sieprawska-Lupa, K. O. Stetter, W. B. Whitman, and D. R. Boone Methanococcus aeolicus sp. nov., a mesophilic, methanogenic archaeon from shallow and deep marine sediments. Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1525 - 1529. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
