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1 National Centre for Animal and Plant Health (CENSA), Apdo 10, San José de Las Lajas, Havana, Cuba
2 CAI "Osvaldo Sánchez", Güines, Havana, Cuba
3 National Institute of Sugarcane Research (INICA), Havana, Cuba
4 Department of Biodiversity and Taxonomy, National Museums and Galleries of Wales, Cardiff, UK
5 Plant-Pathogen Interactions Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
Correspondence
Yaima Arocha
yaimaarocha{at}yahoo.es
During 2003, surveys of sugarcane yellow leaf disease and papaya bunchy top-like disease were carried out on plantations in Havana province, Cuba, to determine the roles of weeds and Auchenorrhyncha insects in the epidemiology of these diseases. More than 250 plant and insect samples were collected and indexed by using a nested PCR for phytoplasma 16S rDNA with the generic primer pairs P1/P7 and R16F2n/R16R2. The PCR products were further characterized by restriction fragment length polymorphism using HaeIII, AluI, Sau3AI, Tru9I, HhaI, HpaII and TaqI endonucleases, giving patterns that distinguished them from those of the other reference phytoplasmas analysed. Phylogenetic analysis of 16S rRNA gene sequences identified the phytoplasmas present in sugarcane (Saccharum officinarum L.), Cynodon dactylon L., Conyza canadensis L. Cronq., Sorghum halepense L. Pers., Macroptilium lathyroides L. Urb., Saccharosydne saccharivora (Westwood) and Cedusa spp., and those in papaya (Carica papaya L.) and Empoasca papayae, as two novel provisional phytoplasma species. We propose that these phytoplasmas should be given Candidatus status, as ‘Candidatus Phytoplasma graminis' and ‘Candidatus Phytoplasma caricae’, respectively.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences reported in this paper are given in Table 1.
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