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Reclassification of Alcaligenes latus strains IAM 12599^T and IAM 12664 and Pseudomonas saccharophila as Azohydromonas lata gen. nov., comb. nov., Azohydromonas australica sp. nov. and Pelomonas saccharophila gen. nov., comb. nov., respectively

Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan

Correspondence
Cheng-Hui Xie
aa37116{at}mail.ecc.u-tokyo.ac.jp

The aim of this study was to clarify the taxonomic position of the nitrogen-fixing and hydrogen-oxidizing bacteria Alcaligenes latus strains IAM 12599^T, IAM 12664 and IAM 12665 and Pseudomonas saccharophila IAM 14368^T. It was found that the type strain of Alcaligenes latus, IAM 12599^T, showed 99·9 and 96·1 % 16S rRNA gene sequence similarity to strains IAM 12665 and IAM 12664, respectively. A comparison using DNA–DNA hybridization suggested that strains IAM 12599^T and IAM 12665 belong to a single species (89·7 %) and that strain IAM 12664 (35·1 %) forms a separate species. The phenotypic characteristics also support the conclusion that these bacteria should be identified as two species of a new genus: Azohydromonas lata gen. nov., comb. nov. (type strain IAM 12599^T=DSM 1122^T=LMG 3321^T=ATCC 29712^T; reference strain IAM 12665=DSM 1123=LMG 3325=ATCC 29714) and Azohydromonas australica sp. nov. (type strain IAM 12664^T=DSM 1124^T=LMG 3324^T=ATCC 29713^T). Pseudomonas saccharophila IAM 14368^T was found to be closely related to the phototrophic bacterium Roseateles depolymerans, with 96·8 % 16S rRNA gene sequence similarity, but the two bacteria are quite different with respect to their metabolism and some significant phenotypic characteristics, suggesting that they cannot be included in a single genus. Further studies on their nifH gene sequences, G+C content of the DNA and cellular fatty acid composition confirm that Pseudomonas saccharophila should be reclassified: the name Pelomonas saccharophila gen. nov., comb. nov. is proposed, with the type strain IAM 14368^T (=LMG 2256^T=ATCC 15946^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Alcaligenes latus strains IAM 12599^T, IAM 12664 and IAM 12665 are AB188125, AB188124 and AB201626, those of the nifH sequences of Rubrivivax gelatinosus IAM 14808^T, Pseudomonas saccharophila IAM 14368^T, Alcaligenes latus IAM 12664 and IAM 12599^T and Derxia gummosa IAM 13946^T are AB188119–AB188123 and that of the nifH sequence of Alcaligenes latus IAM 12665 is AB201627.

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