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Int J Syst Evol Microbiol 55 (2005), 2365-2370; DOI  10.1099/ijs.0.63301-0
© 2005 International Union of Microbiological Societies

Description of Gluconacetobacter swingsii sp. nov. and Gluconacetobacter rhaeticus sp. nov., isolated from Italian apple fruit

Franco Dellaglio1, Ilse Cleenwerck2, Giovanna E. Felis1, Katrien Engelbeen2, Danielle Janssens2 and Marta Marzotto1

1 Science and Technology Department, University of Verona, Strada le Grazie, 15, 37134 Verona, Italy
2 BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium

Correspondence
Franco Dellaglio
franco.dellaglio{at}univr.it

Two Gram-negative, rod-shaped, non-spore-forming bacteria (DST GL01T and DST GL02T) were isolated from apple fruit juice in the region of the Italian Alps. On the basis of 16S rRNA gene sequence similarities, strains DST GL01T and DST GL02T were shown to belong to the {alpha}-subclass of the Proteobacteria, and, in particular, to the genus Gluconacetobacter, in the Gluconacetobacter xylinus branch (98·5–100 %). Chemotaxonomic data (major ubiquinone, Q10; predominant fatty acid, C18 : 1{omega}7c, accounting for approximately 50 % of the fatty acid content) support the affiliation of both strains to the genus Gluconacetobacter. The results of DNA–DNA hybridizations, together with physiological and biochemical data, allowed genotypic and phenotypic differentiation between strains DST GL01T and DST GL02T and from the 11 validly published Gluconacetobacter species. They therefore represent two new species, for which the names Gluconacetobacter swingsii sp. nov. and Gluconacetobacter rhaeticus sp. nov. are proposed, with the type strains DST GL01T (=LMG 22125T=DSM 16373T) and DST GL02T (=LMG 22126T=DSM 16663T), respectively.


The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Gluconacetobacter swingsii DST GL01T and Gluconacetobacter rhaeticus DST GL02T are AY180960 and AY180961, respectively.

Tables of DNA–DNA hybridization data and additional distinguishing characteristics are available as supplementary material in IJSEM Online.




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