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Sphingomonas yunnanensis sp. nov., a novel Gram-negative bacterium from a contaminated plate

Yu-Qin Zhang, Yi-Guang Chen, Wen-Jun Li, Xin-Peng Tian, Li-Hua Xu and Cheng-Lin Jiang

The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, PR China

Correspondence
Wen-Jun Li
wjli{at}ynu.edu.cn or
lihxu{at}ynu.edu.cn

A Gram-negative bacterium, YIM 003^T, which was isolated from a contaminated plate in the laboratory, was subjected to a polyphasic taxonomic study. The organism had short-rod-shaped, motile cells, formed yellow-pigmented colonies on ISP2 medium and its optimum growth pH was 7·0–7·5. The major respiratory lipoquinone was ubiquinone Q-10. The phosphate-containing lipids detected in strain YIM 003^T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and one unidentified phospholipid. The major fatty acids were C_{18 : 1}7c (59·8 %), C_{16 : 0} (9·9 %), ai-C_{17 : 0} (5·3 %), i-C_{17 : 0} (4·4 %) and C_{14 : 0} 2-OH (15·8 %). The G+C content of the genomic DNA was 67·5 mol%. Strain YIM 003^T exhibited levels of 16S rRNA gene sequence similarity of 98·2 % to Sphingomonas phyllosphaerae FA2^T and 98·0 % to Sphingomonas adhaesiva DSM 7418^T but showed less than 97·0 % similarity with respect to other species with validly published names. The DNA–DNA relatedness values of the isolate with S. phyllosphaerae FA2^T and S. adhaesiva DSM 7418^T were 59 and 26 %, respectively. The phenotypic characteristics and genotypic data indicate that strain YIM 003^T should be distinguished from S. phyllosphaerae FA2^T and S. adhaesiva DSM 7418^T. Therefore, on the basis of the polyphasic taxonomic data presented, a novel species of the genus Sphingomonas, Sphingomonas yunnanensis sp. nov., is proposed, with the type strain YIM 003^T (=CCTCC AB 204064^T=KCTC 12346^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 003^T is AY894691.

A two-dimensional thin-layer chromatogram of the phosphate-containing lipids of strain YIM 003^T is available as a supplementary figure in IJSEM Online.

Present address: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.

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