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Shewanella pneumatophori sp. nov., an eicosapentaenoic acid-producing marine bacterium isolated from the intestines of Pacific mackerel (Pneumatophorus japonicus)

¹ Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
² Laboratory of Electron Microscopy, Graduate School of Dentistry, Hokkaido University, Kita-ku, Sapporo 060-8586, Japan
³ Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan
⁴ Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan

Correspondence
Isao Yumoto
i.yumoto{at}aist.go.jp

An eicosapentaenoic acid-producing bacterium, previously described as Shewanella sp. strain SCRC-2738, was classified by phenotypic characterization, chemotaxonomic analysis, 16S rRNA gene sequence analysis and DNA–DNA hybridization. The isolate was Gram-negative, rod-shaped and motile by using polar flagella. The strain grew at 4–32 °C; the optimum growth temperature was 27 °C. NaCl was required for growth. The major isoprenoid quinones were ubiquinone-7 and ubiquinone-8 and its DNA G+C content was 42·8 mol%. The whole-cell fatty acids mainly (above 5 %) consisted of iso-C_{13 : 0}, iso-C_{15 : 0}, C_{16 : 0}, C_{16 : 1}7c, C_{18 : 1}7c and C_{20 : 5}3 (eicosapentaenoic acid). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCRC-2738^T was related closely (sequence similarities above 99 %) to Shewanella marinintestina (99·3 %), Shewanella sairae (99·3 %) and Shewanella schlegeliana (99·2 %). DNA–DNA hybridization and phenotypic characteristics confirmed that strain SCRC-2738^T merited classification as a novel species of the genus Shewanella, for which the name Shewanella pneumatophori sp. nov. is proposed. The type strain is SCRC-2738^T (=JCM 13187^T=NCIMB 14060^T).

Published online ahead of print on 24 June 2005 as DOI 10.1099/ijs.0.63804-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SCRC-2738^T is AB204519.

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