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Methylosarcina lacus sp. nov., a methanotroph from Lake Washington, Seattle, USA, and emended description of the genus Methylosarcina

¹ Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA
² Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195, USA
³ Department of Biology, Pacific Lutheran University, Tacoma, WA 98447, USA
⁴ Department of Microbiology, University of Washington, Seattle, WA 98195, USA

Correspondence
Ludmila Chistoserdova
milachis{at}u.washington.edu

An obligately methanotrophic bacterial strain, LW14^T, isolated from the sediment of Lake Washington, Seattle, USA, is described taxonomically. The isolate is an aerobic, Gram-negative, non-motile bacterium capable of growth on methane, and possesses type I intracytoplasmic membranes (i.e. it is a type I methanotroph). The strain possesses particulate methane monooxygenase (MMO) and has no soluble MMO. Formaldehyde is assimilated via the ribulose monophosphate cycle. The isolate grows within a pH range of 4–8, with the optimum between pH 5·5 and 6·5. The cellular fatty acid profile is dominated by C_{16 :} 18c, C_{16 : 1}7c and C_{16 : 1}5t fatty acids. The DNA G+C content is 53·3±0·4 mol%. On the basis of sequence analysis of the 16S rRNA gene, isolate LW14^T is related most closely to representatives of the genus Methylosarcina. However, DNA–DNA hybridization analysis reveals only a distant relationship between isolate LW14^T and the previously described Methylosarcina species. On the basis of its phenotypic and genotypic characteristics, LW14^T represents a novel species of the genus Methylosarcina, for which the name Methylosarcina lacus sp. nov. is proposed, with LW14^T (=ATCC BAA-1047^T=JCM 13284^T) as the type strain.

Published online ahead of print on 1 July 2005 as DOI 10.1099/ijs.0.63405-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LW14^T is AY007296.

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