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Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species

Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan

Correspondence
Yuichi Nogi
nogiy{at}jamstec.go.jp

Twenty alkaliphilic bacterial strains from industrial applications or enzyme studies were subjected to a polyphasic taxonomic investigation, including 16S rRNA gene sequencing, determination of genomic DNA G+C content, DNA–DNA hybridization, fatty acid analysis and standard bacteriological characterization. By comparing the groupings obtained based on the genomic DNA G+C content and the construction of a phylogenetic tree based on the 16S rRNA gene sequence, 12 clusters of similar strains were recognized. DNA–DNA hybridization revealed that these clusters represented five novel genospecies. Further analysis supported the proposal of five novel species in the genus Bacillus: Bacillus wakoensis sp. nov. (type strain N-1^T=JCM 9140^T=DSM 2521^T), Bacillus hemicellulosilyticus sp. nov. (type strain C-11^T=JCM 9152^T=DSM 16731^T), Bacillus cellulosilyticus sp. nov. (type strain N-4^T=JCM 9156^T=DSM 2522^T), Bacillus akibai sp. nov. (type strain 1139^T=JCM 9157^T=ATCC 43226^T) and Bacillus mannanilyticus sp. nov. (type strain AM-001^T=JCM 10596^T=DSM 16130^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Bacillus wakoensis JCM 9140^T, Bacillus hemicellulosilyticus JCM 9152^T, Bacillus cellulosilyticus JCM 9156^T, Bacillus akibai JCM 9157^T and Bacillus mannanilyticus JCM 10596^T are AB043851, AB043846, AB043852, AB043858 and AB043864, respectively.

Tables detailing the growth characters, the utilization of carbohydrate substrates and fatty acid profiles of the five novel species are available as supplementary material in IJSEM Online.

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