HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dimitriu, P. A. Articles by Mormile, M. R. Search for Related Content PubMed PubMed Citation Articles by Dimitriu, P. A. Articles by Mormile, M. R. Agricola Articles by Dimitriu, P. A. Articles by Mormile, M. R.

Nitrincola lacisaponensis gen. nov., sp. nov., a novel alkaliphilic bacterium isolated from an alkaline, saline lake

Pedro A. Dimitriu^1,, Sanjay K. Shukla², Jennifer Conradt², M. Carmen Márquez³, Antonio Ventosa³, Anne Maglia¹, Brent M. Peyton⁴, Holly C. Pinkart⁵ and Melanie R. Mormile¹

¹ Department of Biological Sciences, University of Missouri-Rolla, Rolla, MO 65401, USA
² Marshfield Clinic Research Foundation, 1000 N. Oak Avenue, Marshfield, WI 54449, USA
³ Department of Microbiology and Parasitology, University of Sevilla, 41012 Sevilla, Spain
⁴ Department of Chemical Engineering, Washington State University, Pullman, WA 99164, USA
⁵ Department of Biological Sciences, Central Washington University, Ellensburg, WA 98926, USA

Correspondence
Melanie R. Mormile
mmormile{at}umr.edu

A novel alkaliphilic bacterium, strain 4CA^T, was isolated from decomposing wood taken from the shore of Soap Lake, a saline, alkaline lake in Grant County, WA, USA. Cells of the isolate were Gram-negative, asporogenous, short, motile rods that utilized only a limited range of organic acids as sole carbon and energy sources. In addition to oxygen, the strain possessed the ability to reduce in the presence of acetate. Strain 4CA^T was oxidase- and catalase-positive; it degraded Tween 60, but not DNA, urea, gelatin or starch. It grew at pH values from 7·5 to 11·0, with optimum growth occurring at pH 9·0, and growth was observed in NaCl concentrations of 0·2–1·3 M, with optimum growth at 0·8 M NaCl. The optimum temperature for growth was 37 °C. Strain 4CA^T was resistant to erythromycin, bacitracin, novobiocin, polymyxin B, neomycin, gentamicin, streptomycin, carbenicillin, rifampicin and tetracycline, and was susceptible to nalidixic acid, chloramphenicol, ampicillin and penicillin. The isolate's 16S rRNA gene sequence indicated that it belonged to the -Proteobacteria, showing 90–94 % similarity to its closest relatives. Maximum-likelihood phylogenetic inferences placed strain 4CA^T within a novel lineage related to the marine bacterial genera Neptunomonas and Marinobacterium. The DNA G+C content of the isolate was 47·4 mol%. On the basis of genotypic and phenotypic characterization, it was concluded that strain 4CA^T should be placed in a separate taxon as a novel genus and species, with the proposed name Nitrincola lacisaponensis gen. nov., sp. nov. The type strain is 4CA^T (=ATCC BAA-920^T=DSM 16316^T).

Published online ahead of print on 3 June 2005 as DOI 10.1099/ijs.0.63647-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Nitrincola lacisaponensis 4CA^T is AY567473.

Present address: Department of Forest Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.

This article has been cited by other articles:

				P. A. Dimitriu, H. C. Pinkart, B. M. Peyton, and M. R. Mormile Spatial and Temporal Patterns in the Microbial Diversity of a Meromictic Soda Lake in Washington State Appl. Envir. Microbiol., August 1, 2008; 74(15): 4877 - 4888. [Abstract] [Full Text] [PDF]