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Planifilum fimeticola gen. nov., sp. nov. and Planifilum fulgidum sp. nov., novel members of the family ‘Thermoactinomycetaceae’ isolated from compost

¹ Division of Integrative Environmental Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan
² Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan
³ Institute of Hyperthermophiles, Motobu-Noge Hospital, Aza-Ohama 880-1, Motobu, Okinawa 905-0212, Japan

Correspondence
Akira Nakamura
a-nak{at}agbi.tsukuba.ac.jp

Four thermophilic, Gram-positive strains, designated H0165^T, 500275^T, C0170 and 700375, were isolated from a composting process in Japan. The isolates grew aerobically at about 65 °C on a solid medium with formation of substrate mycelia; spores were produced singly along the mycelia. These morphological characters resembled those of some type strains of species belonging to the family ‘Thermoactinomycetaceae’, except that aerial mycelia were not formed. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the closest related species to the isolates were members of the family ‘Thermoactinomycetaceae’, but that the isolates formed an independent phylogenetic lineage. Some chemotaxonomic characters of the isolates, such as DNA G+C contents of 58·7–60·3 mol%, MK-7 as the major menaquinone and cellular fatty acid profiles, differed from those of members of the family ‘Thermoactinomycetaceae’. DNA–DNA hybridization showed that the isolates could be divided into two genomic groups, strain H0165^T and the other three strains. These results indicated that the four isolates should be classified into two species of a novel genus in the family ‘Thermoactinomycetaceae’, for which the names Planifilum fimeticola gen. nov., sp. nov. (type strain H0165^T=ATCC BAA-969^T=JCM 12507^T) and Planifilum fulgidum sp. nov. (type strain 500275^T=ATCC BAA-970^T=JCM 12508^T) are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Planifilum fimeticola H0165^T and Planifilum fulgidum 500275^T, C0170 and 700375 are AB088364, AB088362, AB088363 and AB088361, respectively.

Tables detailing levels of DNA–DNA relatedness among the novel strains and cellular fatty acid profiles of the four strains plus representative members of the family ‘Thermoactinomycetaceae’ are available as supplementary material in IJSEM Online.

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