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Thermincola carboxydiphila gen. nov., sp. nov., a novel anaerobic, carboxydotrophic, hydrogenogenic bacterium from a hot spring of the Lake Baikal area

Institute of Microbiology, Russian Academy of Sciences, Prospekt 60, let Oktyabrya 7/2, 117811 Moscow, Russia

Correspondence
Tatyana Sokolova
tatso{at}mail.ru

A novel anaerobic, thermophilic, alkalitolerant bacterium, strain 2204^T, was isolated from a hot spring of the Baikal Lake region. The cells of strain 2204^T were straight rods of variable length, Gram-positive with an S-layer, motile with one to two lateral flagella, and often formed aggregates of 3–15 cells. The isolate was shown to be an obligate anaerobe oxidizing CO and producing equimolar quantities of H₂ and CO₂ according to the equation CO+H₂OCO₂+H₂. No organic substrates were used as energy sources. For lithotrophic growth on CO, 0·2 g acetate or yeast extract l^–1 was required but did not support growth in the absence of CO. Growth was observed in the temperature range 37–68 °C, the optimum being 55 °C. The pH range for growth was 6·7–9·5, the optimum pH being 8·0. The generation time under optimal conditions was 1·3 h. The DNA G+C content was 45 mol%. Penicillin, erythromycin, streptomycin, rifampicin, vancomycin and tetracycline completely inhibited both growth and CO utilization by strain 2204^T. Thus, isolate 2204^T was found to be the first known moderately thermophilic and alkalitolerant H₂-producing anaerobic carboxydotroph. The novel bacterium fell within the cluster of the family Peptococcaceae within the low-G+C-content Gram-positive bacteria, where it formed a separate branch. On the basis of morphological, physiological and phylogenetic features, strain 2204^T should be assigned to a novel genus and species, for which the name Thermincola carboxydiphila gen. nov., sp. nov. is proposed. The type strain is strain 2204^T (=DSM 17129^T=VKM B-2283^T=JCM 13258^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Thermincola carboxydiphila strain 2204^T is AY603000.

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