HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pal, R. Articles by Lal, R. Search for Related Content PubMed PubMed Citation Articles by Pal, R. Articles by Lal, R. Agricola Articles by Pal, R. Articles by Lal, R.

Hexachlorocyclohexane-degrading bacterial strains Sphingomonas paucimobilis B90A, UT26 and Sp+, having similar lin genes, represent three distinct species, Sphingobium indicum sp. nov., Sphingobium japonicum sp. nov. and Sphingobium francense sp. nov., and reclassification of [Sphingomonas] chungbukensis as Sphingobium chungbukense comb. nov.

¹ Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi – 110007, India
² Center for Cellular and Molecular Biology, Hyderabad – 500007, India
³ Department of Genetics, University of Kaiserslautern, 67663 Kaiserslautern, Germany
⁴ EPFL, ENAC-ISTE, Laboratory of Environmental Biotechnology, CH 1015 Lausanne, Switzerland

Correspondence
Rup Lal
duzdel{at}vsnl.com

Three strains of Sphingomonas paucimobilis, B90A, UT26 and Sp+, isolated from different geographical locations, were found to degrade hexachlorocyclohexane. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains do not fall in a clade that includes the type strain, Sphingomonas paucimobilis ATCC 29837^T, but form a coherent cluster with [Sphingomonas] chungbukensis IMSNU 11152^T followed by Sphingobium chlorophenolicum ATCC 33790^T. The three strains showed low DNA–DNA relatedness values with Sphingomonas paucimobilis ATCC 29837^T (8–25 %), [Sphingomonas] chungbukensis IMSNU 11152^T (10–17 %), Sphingobium chlorophenolicum ATCC 33790^T (23–54 %) and Sphingomonas xenophaga DSM 6383^T (10–28 %), indicating that they do not belong to any of these species. Although the three strains were found to be closely related to each other based on 16S rRNA gene sequence similarity (99·1–99·4 %), DNA–DNA relatedness (19–59 %) and pulsed-field gel electrophoresis (PFGE) patterns indicated that they possibly represent three novel species of the genus Sphingobium. The three strains could also be readily distinguished by biochemical tests. The three strains showed similar polar lipid profiles and contained sphingoglycolipids. The strains differed from each other in fatty acid composition but contained the predominant fatty acids characteristic of other Sphingobium species. A phylogenetic study based on 16S rRNA gene sequences showed that [Sphingomonas] chungbukensis IMSNU 11152^T formed a cluster with members of the genus Sphingobium. Based on these results, it is proposed that strains B90A, UT26 and Sp+, previously known as Sphingomonas paucimobilis, are the type strains of Sphingobium indicum sp. nov. (=MTCC 6364^T=CCM 7286^T), Sphingobium japonicum sp. nov. (=MTCC 6362^T=CCM 7287^T) and Sphingobium francense sp. nov. (=MTCC 6363^T=CCM 7288^T), respectively. It is also proposed that [Sphingomonas] chungbukensis be transferred to Sphingobium chungbukense comb. nov.

Published online ahead of print on 29 April 2005 as DOI 10.1099/ijs.0.63201-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Sphingobium indicum sp. nov. MTCC 6364^T and Sphingobium francense sp. nov. MTCC 6363^T are AY519129 and AY519130, respectively.

A table showing DNA–DNA relatedness values for strains B90A, UT26, Sp+ and Sphingomonas paucimobilis ATCC 29837^T is available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				H. Kumari, S. K. Gupta, S. Jindal, P. Katoch, and R. Lal Sphingobium lactosutens sp. nov., isolated from a hexachlorocyclohexane dump site and Sphingobium abikonense sp. nov., isolated from oil-contaminated soil Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2291 - 2296. [Abstract] [Full Text] [PDF]

				S. K. Gupta, D. Lal, and R. Lal Novosphingobium panipatense sp. nov. and Novosphingobium mathurense sp. nov., from oil-contaminated soil Int J Syst Evol Microbiol, January 1, 2009; 59(1): 156 - 161. [Abstract] [Full Text] [PDF]

				A. Singh and R. Lal Sphingobium ummariense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium, isolated from HCH-contaminated soil Int J Syst Evol Microbiol, January 1, 2009; 59(1): 162 - 166. [Abstract] [Full Text] [PDF]

				C.-C. Young, A. B. Arun, P. Kampfer, H.-J. Busse, W.-A. Lai, W.-M. Chen, F.-T. Shen, and P. D. Rekha Sphingobium rhizovicinum sp. nov., isolated from rhizosphere soil of Fortunella hindsii (Champ. ex Benth.) Swingle Int J Syst Evol Microbiol, August 1, 2008; 58(8): 1801 - 1806. [Abstract] [Full Text] [PDF]

				S. K. Gupta, R. Kumari, O. Prakash, and R. Lal Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site Int J Syst Evol Microbiol, June 1, 2008; 58(6): 1339 - 1345. [Abstract] [Full Text] [PDF]

				C. C. Young, M.-J. Ho, A. B. Arun, W.-M. Chen, W.-A. Lai, F.-T. Shen, P. D. Rekha, and A. F. Yassin Sphingobium olei sp. nov., isolated from oil-contaminated soil Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2613 - 2617. [Abstract] [Full Text] [PDF]

				G. S. N. Reddy and F. Garcia-Pichel Sphingomonas mucosissima sp. nov. and Sphingomonas desiccabilis sp. nov., from biological soil crusts in the Colorado Plateau, USA Int J Syst Evol Microbiol, May 1, 2007; 57(5): 1028 - 1034. [Abstract] [Full Text] [PDF]

				O. Prakash, K. Kumari, and R. Lal Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant Int J Syst Evol Microbiol, March 1, 2007; 57(3): 527 - 531. [Abstract] [Full Text] [PDF]

				R.-M. Wittich, H.-J. Busse, P. Kampfer, M. Tiirola, M. Wieser, A. J. Macedo, and W.-R. Abraham Sphingobium aromaticiconvertens sp. nov., a xenobiotic-compound-degrading bacterium from polluted river sediment Int J Syst Evol Microbiol, February 1, 2007; 57(2): 306 - 310. [Abstract] [Full Text] [PDF]

				B. Geueke, H.-J. Busse, T. Fleischmann, P. Kampfer, and H.-P. E. Kohler Description of Sphingosinicella xenopeptidilytica sp. nov., a beta-peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans Int J Syst Evol Microbiol, January 1, 2007; 57(1): 107 - 113. [Abstract] [Full Text] [PDF]

				P. Sharma, V. Raina, R. Kumari, S. Malhotra, C. Dogra, H. Kumari, H.-P. E. Kohler, H.-R. Buser, C. Holliger, and R. Lal Haloalkane Dehalogenase LinB Is Responsible for {beta}- and {delta}-Hexachlorocyclohexane Transformation in Sphingobium indicum B90A Appl. Envir. Microbiol., September 1, 2006; 72(9): 5720 - 5727. [Abstract] [Full Text] [PDF]

				O. Prakash and R. Lal Description of Sphingobium fuliginis sp. nov., a phenanthrene-degrading bacterium from a fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov. Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2147 - 2152. [Abstract] [Full Text] [PDF]

				R. Pal, V. K. Bhasin, and R. Lal Proposal to reclassify [Sphingomonas] xenophaga Stolz et al. 2000 and [Sphingomonas] taejonensis Lee et al. 2001 as Sphingobium xenophagum comb. nov. and Sphingopyxis taejonensis comb. nov., respectively. Int J Syst Evol Microbiol, March 1, 2006; 56(Pt 3): 667 - 670. [Abstract] [Full Text] [PDF]