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Staphylococcus simiae sp. nov., isolated from South American squirrel monkeys

Roman Pantek¹, Ivo Sedláek², Petr Petrá³, Dagmar Koukalová⁴, Pavel vec², Vlastimil ttina², Marc Vancanneyt⁵, Lenka Chrastinová⁶, Jitka Vokurková⁶, Vladislava Riková¹, Jií Doka¹, Jean Swings⁵ and Václav Hájek⁴

¹ Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University Brno, Kotláská 2, 611 37 Brno, Czech Republic
² Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic
³ Reference Laboratory for Staphylococci, National Institute of Public Health, robárova 48, 100 42 Prague, Czech Republic
⁴ Institute of Microbiology, Faculty of Medicine, Palack University, Hnvotínská 3, 775 15 Olomouc, Czech Republic
⁵ BCCM/LMG Bacteria Collection, Ghent University, K. L. Ledeganckstraat 35, B–9000 Ghent, Belgium
⁶ Zoo Olomouc, Darwinova 29, 772 00 Olomouc, Czech Republic

Correspondence
Ivo Sedláek
ivo{at}sci.muni.cz

Eight coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococcal strains were isolated from the gastrointestinal tracts of South American squirrel monkeys (Saimiri sciureus L.). These strains were differentiated from known staphylococcal species on the basis of 16S rRNA gene and hsp60 gene sequencing, and from the most closely related species by using DNA–DNA hybridization, ribotyping, whole-cell protein profiles and biotyping. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that these strains are members of the Staphylococcus aureus species group (99 % similarity) but are biochemically similar to Staphylococcus piscifermentans, from which they can be phenotypically distinguished by resistance to polymyxin B, acid production from D-mannitol, the inability to hydrolyse aesculin and DNA and the absence of -glucosidase. On the basis of these analyses, a novel species of the genus Staphylococcus is described, for which the name Staphylococcus simiae sp. nov. is proposed, with CCM 7213^T (=LMG 22723^T) as the type strain.

Published online ahead of print on 29 April 2005 as DOI 10.1099/ijs.0.63590-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and the partial hsp60 gene sequences of strain CCM 7213^T are AY727530 and AY727531.

SDS-PAGE whole-cell protein profiles, ribotype patterns, internal transcribed ribosomal spacer-PCR patterns and PFGE macrorestriction patterns of the novel isolates and reference Staphylococcus species are available as supplementary figures in IJSEM Online.

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