HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Tables Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sakamoto, M. Articles by Benno, Y. Search for Related Content PubMed PubMed Citation Articles by Sakamoto, M. Articles by Benno, Y. Agricola Articles by Sakamoto, M. Articles by Benno, Y.

Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque

¹ Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan
² Division of Periodontology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8549, Japan

Correspondence
Mitsuo Sakamoto
sakamoto{at}jcm.riken.jp

Six bacterial strains isolated from the human oral cavity, PPPA16, PPPA20^T, PPPA24, PPPA31, EPPA6 and EPPA7, were characterized by determining phenotypic and biochemical features, cellular fatty acid profiles, menaquinone profiles and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that the isolates represented the same species of the genus Prevotella. The strains were related to Prevotella dentalis with about 89 % similarity. In addition, the isolates were related to Prevotella sp. oral clone IDR-CEC-0032, which is a representative of the numerically dominant cluster VI in carious dentine lesions [Nadkarni et al. (2004). J Clin Microbiol 42, 5238–5244], with about 99 % similarity. The strains were obligately anaerobic, non-pigmenting, non-spore-forming, non-motile, Gram-negative rods. The isolates could be differentiated from other Prevotella species by D-mannitol, D-melezitose, D-sorbitol and D-trehalose fermentation in API 20A tests. The cellular fatty acid composition of strains PPPA16, PPPA20^T, PPPA24, PPPA31, EPPA6 and EPPA7 was significantly different from that of other Prevotella species. Compared with other Prevotella species, only these six strains contained dimethyl acetals. The major menaquinones of the clinical isolates were MK-12 and MK-13, whereas the major menaquinones of other Prevotella species were MK-10 and MK-11. On the basis of these data, a novel Prevotella species, Prevotella multisaccharivorax sp. nov., is proposed, with PPPA20^T (=JCM 12954^T=DSM 17128^T) as the type strain.

Published online ahead of print on 15 April 2005 as DOI 10.1099/ijs.0.63739-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of P. multisaccharivorax strain PPPA20^T is AB200414.

Phenotypic characteristics, cellular fatty acid composition and API ZYM and API An-Ident test results are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				J. Downes, M. Liu, E. Kononen, and W. G. Wade Prevotella micans sp. nov., isolated from the human oral cavity Int J Syst Evol Microbiol, April 1, 2009; 59(4): 771 - 774. [Abstract] [Full Text] [PDF]

				M. Sakamoto, H. Kumada, N. Hamada, Y. Takahashi, M. Okamoto, M. A. Bakir, and Y. Benno Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque Int J Syst Evol Microbiol, February 1, 2009; 59(2): 319 - 322. [Abstract] [Full Text] [PDF]

				P. A. Lawson, E. Moore, and E. Falsen Prevotella amnii sp. nov., isolated from human amniotic fluid Int J Syst Evol Microbiol, January 1, 2008; 58(1): 89 - 92. [Abstract] [Full Text] [PDF]

				M. Sakamoto, K. Ohkusu, T. Masaki, H. Kako, T. Ezaki, and Y. Benno Prevotella pleuritidis sp. nov., isolated from pleural fluid Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1725 - 1728. [Abstract] [Full Text] [PDF]

				A. Ueki, H. Akasaka, A. Satoh, D. Suzuki, and K. Ueki Prevotella paludivivens sp. nov., a novel strictly anaerobic, Gram-negative, hemicellulose-decomposing bacterium isolated from plant residue and rice roots in irrigated rice-field soil Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1803 - 1809. [Abstract] [Full Text] [PDF]

				H. Hayashi, K. Shibata, M. Sakamoto, S. Tomita, and Y. Benno Prevotella copri sp. nov. and Prevotella stercorea sp. nov., isolated from human faeces Int J Syst Evol Microbiol, May 1, 2007; 57(5): 941 - 946. [Abstract] [Full Text] [PDF]