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Genomovars 11 to 18 of Pseudomonas stutzeri, identified among isolates from soil and marine sediment

Johannes Sikorski^1,, Jorge Lalucat² and Wilfried Wackernagel¹

¹ Genetik, Institut für Biologie und Umweltwissenschaften, Universität Oldenburg, Germany
² Microbiologia, Departament de Biologia, Institut Mediterrani d'Estudis Avancats (CSICUIB), Palma de Mallorca, Spain

Correspondence
Johannes Sikorski
sikorski{at}research.haifa.ac.il

Amongst 440 strains of Pseudomonas stutzeri isolated from soil and marine sediment for a population genetic study, eight strains were each presumed to represent a novel genomic group and were compared with each other and to reference strains of P. stutzeri genomovars 1 to 10 and other Pseudomonas species by DNA–DNA hybridization, 16S rRNA and internally transcribed 16S–23S rRNA spacer region (ITS1) sequences and basic physiological properties defining the species. While 16S rRNA and ITS1 gene sequences positioned the eight strains within the phylogenetic branch of P. stutzeri, the DNA–DNA hybridizations with reference strains of the 10 described genomovars and among the novel strains were generally below 70 %, which is the threshold for species and genomovar differentiation. Since the physiological properties studied in the eight strains fitted the profile of P. stutzeri, eight new genomovars of P. stutzeri, numbered 11 to 18, are proposed, with strains 28a50, 28a39, 28a22, 28a3, 4C29, 24a13, 24a75 and MT-1 being the reference strains. The highly transformable reference strain 28a3 of genomovar 14 had a localized 16S rRNA gene sequence tag characteristic of genomovar strains 2 and 3, suggesting a possible horizontal gene transfer event involving part of the 16S rRNA gene.

Published online ahead of print on 22 April 2005 as DOI 10.1099/ijs.0.63535-0.

The GenBank/EMBL/DDBJ accession numbers for the ITS1 rRNA gene sequences of genomovars 11 to 18 of Pseudomonas stutzeri are AY850023–AY850031.

Two phylogenetic trees based on 16S rRNA and ITS1 gene sequences and tables detailing DNA–DNA relatedness values and physiological properties of the genomovars are available as supplementary material in IJSEM Online.

Present address: Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel.

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