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Pseudoalteromonas spongiae sp. nov., a novel member of the -Proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters

¹ Coastal Marine Laboratory/Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong
² Department of Biology, Chinese University of Hong Kong, Shatin, N.T., Hong Kong

Correspondence
Pei-Yuan Qian
boqianpy{at}ust.hk

A Gram-negative, non-spore-forming, short rod-shaped bacterium (UST010723-006^T) was isolated from the surface of the sponge Mycale adhaerens in Hong Kong waters. Cells of UST010723-006^T did not have flagella and were non-motile. Colonies were pale orange in colour, 2–4 mm in diameter, convex with a smooth surface and an entire translucent margin. Gas bubbles were observed in the colonies and also in the agar matrix underneath and adjacent to the colonies. UST010723-006^T was heterotrophic, strictly aerobic and required NaCl for growth (2·0–6·0 %). It grew at pH 5·0–10·0 and between 12 and 44 °C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST010723-006^T within the genus Pseudoalteromonas of the -subclass of the Proteobacteria. The DNA G+C content is 40·6 mol% and the dominant fatty acids were 12 : 0 3-OH, 14 : 0, 15 : 0 iso 2-OH, 16 : 0, 16 : 17, 17 : 18 and 18 : 17 (altogether representing 75·9 % of the total).These data supported the affiliation of UST010723-006^T to the genus Pseudoalteromonas. The closest relatives were Pseudoalteromonas luteviolacea, P. phenolica, P. rubra and P. ruthenica with similarity values ranging from 95·4 to 96·8 %. UST010723-006^T differed from these closest relatives by 9–19 traits. Molecular evidence, together with phenotypic characteristics, suggests that UST010723-006^T constitutes a novel species within the genus Pseudoalteromonas. The name Pseudoalteromonas spongiae sp. nov. is proposed for this bacterium. The type strain is UST010723-006^T (=NRRL B-41100^T=JCM 12884^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain UST010723-006^T is AY769918.

Supplementary material available in IJSEM Online comprises a table showing primers used for the construction of 16S rRNA gene sequences, TEM and SEM images and a table detailing the carbohydrate metabolism of strain UST010723-006^T.

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