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Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity

¹ Infection Research Group, Dental Institute, King's College London, London SE1 9RT, UK
² Division of Biomedical Sciences, School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK
³ Department of Molecular Genetics, The Forsyth Institute, Boston, MA 02115, USA

Correspondence
William G. Wade
william.wade{at}kcl.ac.uk

Eleven strains of anaerobic Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise two homogeneous groups, designated E2 and E4. 16S rRNA gene sequence analysis revealed that members of both groups belonged to the genus Prevotella but were distinct from any species with validly published names. This distinction was confirmed by DNA–DNA hybridization and phenotypic tests. Two novel species are therefore proposed: Prevotella marshii sp. nov. (group E2) and Prevotella baroniae (group E4). Both species are saccharolytic; the end-products of fermentation for P. marshii are acetic, propionic and succinic acids, while P. baroniae produces acetic and succinic acids with minor amounts of isovaleric and isobutyric acids. The G+C content of the DNA of the type strain of Prevotella marshii is 51 mol% and that of Prevotella baroniae is 52 mol%. The type strain for P. marshii is E9.34^T (=DSM 16973^T=CCUG 50419^T) and that for P. baroniae is E9.33^T (=DSM 16972^T=CCUG 50418^T).

Published online ahead of print on 1 March 2005 as DOI 10.1099/ijs.0.63634-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of P. marshii E9.34^T, P. baroniae E9.33^T, P. loescheii NCTC 11321^T, P. oralis NCTC 11459^T, P. denticola ATCC 35308^T, P. melaninogenica ATCC 25845^T and P. veroralis ATCC 33779^T are AF481227, AY840553, AY836508, AY323522, AY323524, AY323525 and AY836507, respectively.

Transmission electron micrographs of P. baroniae E9.33^T and P. marshii E9.34^T, an extended phylogenetic tree and details of FAME profiles are available as supplementary material in IJSEM Online.

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