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Shewanella sediminis sp. nov., a novel Na⁺-requiring and hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading bacterium from marine sediment

Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec, Canada H4P 2R2

Correspondence
Jalal Hawari
jalal.hawari{at}cnrc-nrc.gc.ca

Previously, a psychrophilic rod-shaped marine bacterium (strain HAW-EB3^T) isolated from Halifax Harbour sediment was noted for its ability to degrade hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In the present study phenotypic, chemotaxonomic and genotypic characterization showed that strain HAW-EB3^T represents a novel species of Shewanella. Strain HAW-EB3^T contained lysine decarboxylase, which is absent in other known Shewanella species, and distinguished itself from most other species of Shewanella by the presence of arginine dehydrolase, ornithine decarboxylase and chitinase, and by its ability to oxidize and ferment N-acetyl-D-glucosamine. Strain HAW-EB3^T grew on several carbon sources (N-acetyl-D-glucosamine, Tween 40, Tween 80, acetate, succinate, butyrate and serine) and showed distinctive fatty acid and quinone compositions. Both phenotypic and 16S rRNA gene phylogenetic cluster analyses demonstrated that HAW-EB3^T belongs to the Na⁺-requiring group of Shewanella species. The HAW-EB3^T 16S rRNA gene sequence displayed 97·4 % similarity to all known Shewanella species and was most similar to those of two bioluminescent species, Shewanella hanedai and Shewanella woodyi. However, gyrB of strain HAW-EB3^T was significantly different from those of other Shewanella species, with similarities less than 85 %. DNA-DNA hybridization showed that its genomic DNA was less than 25 % related to that of S. hanedai or S. woodyi. Therefore we propose Shewanella sediminis sp. nov., with HAW-EB3^T (=NCIMB 14036^T=DSM 17055^T) as the type strain.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and the gyrB gene sequence of strain HAW-EB3^T are AY579750 and AY842130, respectively.

A table of the quinone compositions of strain HAW-EB3^T and other species of Shewanella is available as supplementary material in IJSEM Online.

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