HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, S. Articles by Dong, X. Search for Related Content PubMed PubMed Citation Articles by Chen, S. Articles by Dong, X. Agricola Articles by Chen, S. Articles by Dong, X.

Syntrophobacter sulfatireducens sp. nov., a novel syntrophic, propionate-oxidizing bacterium isolated from UASB reactors

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China
² Graduate School of the Chinese Academy of Sciences, Beijing 100080, PR China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

Two obligate anaerobes, TB8106^T and WZH410, which degraded propionate in syntrophic association with methanogens, were isolated from two upflow anaerobic sludge blanket reactors, one treating brewery wastewater and the other bean curd wastewater. The strains were Gram-negative, non-spore-forming and non-motile. Cells were egg-shaped, with a size of 1·0–1·3x1·8–2·2 µm. Growth was observed at 20–48 °C and pH 6·2–8·8. Both strains converted propionate to acetate and methane in co-culture with methanogens, and grew on propionate plus sulfate in pure culture, with a doubling time of 52–55 h at 37 °C. Sulfate and thiosulfate both served as electron acceptors for propionate degradation. The DNA G+C contents of the two strains were 58·5 and 58·7 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were closely related to a propionate-oxidizing syntrophic bacterium, Syntrophobacter fumaroxidans DSM 10017^T (94·7 % similarity). However, the novel strains could not ferment fumarate, and grew at a more alkaline pH range than Syntrophobacter fumaroxidans. Moreover, the novel isolates had obviously higher growth rates on propionate plus sulfate (0·12 day^–1) than Syntrophobacter fumaroxidans DSM 10017^T (0·024 day^–1). Therefore, a novel species, Syntrophobacter sulfatireducens sp. nov., is proposed, with strain TB8106^T (=AS 1.5016^T=DSM 16706^T) as the type strain.

Published online ahead of print on 14 January 2005 as DOI 10.1099/ijs.0.63565-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Syntrophobacter sulfatireducens TB8106^T is AY651787.

A transmission electron micrograph of a cell of Syntrophobacter sulfatireducens TB8106^T is available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				H. Imachi, S. Sakai, A. Ohashi, H. Harada, S. Hanada, Y. Kamagata, and Y. Sekiguchi Pelotomaculum propionicicum sp. nov., an anaerobic, mesophilic, obligately syntrophic, propionate-oxidizing bacterium Int J Syst Evol Microbiol, July 1, 2007; 57(7): 1487 - 1492. [Abstract] [Full Text] [PDF]

				H. Imachi, Y. Sekiguchi, Y. Kamagata, A. Loy, Y.-L. Qiu, P. Hugenholtz, N. Kimura, M. Wagner, A. Ohashi, and H. Harada Non-sulfate-reducing, syntrophic bacteria affiliated with desulfotomaculum cluster I are widely distributed in methanogenic environments. Appl. Envir. Microbiol., March 1, 2006; 72(3): 2080 - 2091. [Abstract] [Full Text] [PDF]

				S. Chen and X. Dong Proteiniphilum acetatigenes gen. nov., sp. nov., from a UASB reactor treating brewery wastewater Int J Syst Evol Microbiol, November 1, 2005; 55(6): 2257 - 2261. [Abstract] [Full Text] [PDF]