HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Miller, J. A. Articles by Chistoserdova, L. Search for Related Content PubMed PubMed Citation Articles by Miller, J. A. Articles by Chistoserdova, L. Agricola Articles by Miller, J. A. Articles by Chistoserdova, L.

Labrys methylaminiphilus sp. nov., a novel facultatively methylotrophic bacterium from a freshwater lake sediment

Jonathan A. Miller¹, Marina G. Kalyuzhnaya², Emma Noyes^3,, Jimmie C. Lara¹, Mary E. Lidstrom^1,2 and Ludmila Chistoserdova²

¹ Department of Microbiology, University of Washington, Seattle, WA 98195, USA
² Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA
³ Omak High School, Omak, WA 98841, USA

Correspondence
Ludmila Chistoserdova
milachis{at}u.washington.edu

A new bacterial isolate from a methylamine enrichment culture is described, representing a novel species of facultatively methylotrophic bacteria. The non-motile bacterium is Gram-negative, replicates by budding and does not form endospores. The isolate utilizes methylated amines, as well as a variety of monosaccharides, disaccharides, amino acids, organic acids, aromatic compounds and alcohols as substrates, but does not utilize methanol. Growth factors are not required, although yeast extract stimulates growth. The major components of the fatty acid profile are C_{18 : 1}7c, C_{19 : 0} cyclo and C_{16 : 0}. The dominant cellular phospholipids are phosphatidyl acid, phosphatidylcholine and phosphatidylethanolamine. The G+C content of the DNA is 65·7±0·3 mol%. 16S rRNA gene-based phylogenetic analysis revealed that the novel isolate belongs to the -Proteobacteria and is closely related to the only representative of the genus Labrys, Labrys monachus (97·4 % sequence similarity). However, the level of DNA–DNA relatedness with L. monachus is less than 3 %, justifying the placement of this isolate into a novel species of the genus Labrys. The name Labrys methylaminiphilus sp. nov. is proposed (type strain JLW10^T=ATCC BAA-1080^T=DSM 16812^T).

Published online ahead of print on 14 January 2005 as DOI 10.1099/ijs.0.63409-0.

The GenBank/EMBL/DDBJ accession number for the nearly complete sequence of the 16S rRNA gene of Labrys methylaminiphilus JLW10^T is AY766152.

Present address: Department of Microbiology, University of Washington, Seattle, WA 98195, USA.

This article has been cited by other articles:

				M. G. Kalyuzhnaya, V. Khmelenina, B. Eshinimaev, D. Sorokin, H. Fuse, M. Lidstrom, and Y. Trotsenko Classification of halo(alkali)philic and halo(alkali)tolerant methanotrophs provisionally assigned to the genera Methylomicrobium and Methylobacter and emended description of the genus Methylomicrobium Int J Syst Evol Microbiol, March 1, 2008; 58(3): 591 - 596. [Abstract] [Full Text] [PDF]

				M. F. Carvalho, P. De Marco, A. F. Duque, C. C. Pacheco, D. B. Janssen, and P. M. L. Castro Labrys portucalensis sp. nov., a fluorobenzene-degrading bacterium isolated from an industrially contaminated sediment in northern Portugal Int J Syst Evol Microbiol, March 1, 2008; 58(3): 692 - 698. [Abstract] [Full Text] [PDF]

				C. Y. Hwang and B. C. Cho Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales, and proposal of Cohaesibacteraceae fam. nov. Int J Syst Evol Microbiol, January 1, 2008; 58(1): 267 - 277. [Abstract] [Full Text] [PDF]

				M. S. Islam, H. Kawasaki, Y. Nakagawa, T. Hattori, and T. Seki Labrys okinawensis sp. nov. and Labrys miyagiensis sp. nov., budding bacteria isolated from rhizosphere habitats in Japan, and emended descriptions of the genus Labrys and Labrys monachus Int J Syst Evol Microbiol, March 1, 2007; 57(3): 552 - 557. [Abstract] [Full Text] [PDF]

				Y.-J. Chou, G. N. Elliott, E. K. James, K.-Y. Lin, J.-H. Chou, S.-Y. Sheu, D.-S. Sheu, J. I. Sprent, and W.-M. Chen Labrys neptuniae sp. nov., isolated from root nodules of the aquatic legume Neptunia oleracea Int J Syst Evol Microbiol, March 1, 2007; 57(3): 577 - 581. [Abstract] [Full Text] [PDF]

				M. G. Kalyuzhnaya, S. Bowerman, J. C. Lara, M. E. Lidstrom, and L. Chistoserdova Methylotenera mobilis gen. nov., sp. nov., an obligately methylamine-utilizing bacterium within the family Methylophilaceae Int J Syst Evol Microbiol, December 1, 2006; 56(12): 2819 - 2823. [Abstract] [Full Text] [PDF]

				M. G. Kalyuzhnaya, P. De Marco, S. Bowerman, C. C. Pacheco, J. C. Lara, M. E. Lidstrom, and L. Chistoserdova Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates. Int J Syst Evol Microbiol, November 1, 2006; 56(Pt 11): 2517 - 2522. [Abstract] [Full Text] [PDF]

				P. Kampfer, C.-C. Young, A. B. Arun, F.-T. Shen, U. Jackel, R. Rossello-Mora, W.-A. Lai, and P. D. Rekha Pseudolabrys taiwanensis gen. nov., sp. nov., an alphaproteobacterium isolated from soil. Int J Syst Evol Microbiol, October 1, 2006; 56(Pt 10): 2469 - 2472. [Abstract] [Full Text] [PDF]