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Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa

Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan

Correspondence
Cheng-Hui Xie
aa37116{at}mail.ecc.u-tokyo.ac.jp

Five strains of free-living diazotrophs isolated from rice were characterized by using a polyphasic approach. The strains were found to be very closely related, with 99–100 % 16S rRNA gene sequence similarity and DNA–DNA hybridization values greater than 70 %, suggesting that they represent a single species. When compared with other recognized species, they showed not more than 93 and 89 % similarity for the 16S rRNA and nifH gene sequences, respectively. Phylogenetic distances showed that these isolates were distinct from other taxa within the -Proteobacteria. Chemotaxonomic characteristics of these isolates included the DNA G+C content (62·1–63·1 mol%), the major quinone system (Q-10), predominant fatty acids (18 : 17c, cyclo 19 : 08c and 16 : 0) and major hydroxy fatty acids (14 : 0 3-OH, 18 : 0 3-OH and 16 : 0 3-OH). Based on phylogenetic and phenotypic analyses, these isolates are considered to represent a novel genus and species, for which the name Pleomorphomonas oryzae gen. nov., sp. nov. is proposed. The type strain is F-7^T (=IAM 15079^T=ATCC BAA-940^T=DSM 16300^T).

Published online ahead of print on 7 January 2005 as DOI 10.1099/ijs.0.63406-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains F-7^T, B-24, B-4, B-18 and B-32 are AB159680 and AB159682–AB159685, respectively, and for the nifH gene sequences they are AB161454, AB182912, AB159683, AB182911 and AB182913, respectively.

A neighbour-joining tree based on nifH gene sequences showing the position of the novel strains is available as supplementary material in IJSEM Online.

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