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Int J Syst Evol Microbiol 55 (2005), 1193-1197; DOI  10.1099/ijs.0.63271-0
© 2005 International Union of Microbiological Societies

Clostridium sardiniense Prévot 1938 and Clostridium absonum Nakamura et al. 1973 are heterotypic synonyms: evidence from phylogenetic analyses of phospholipase C and 16S rRNA sequences, and DNA relatedness

Xingmin Wang{dagger}, Tsuneo Maegawa{ddagger}, Tadahiro Karasawa§, Eijiro Ozaki and Shinichi Nakamura

Department of Bacteriology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640, Japan

Correspondence
Tadahiro Karasawa
karasawa{at}mhs.mp.kanazawa-u.ac.jp

Clostridium sardiniense Prévot 1938 and Clostridium absonum Nakamura et al. 1973 have long been considered similar in terms of their biological and biochemical properties, but their taxonomic positions have not been clarified by DNA–DNA hybridization studies or rigorous analysis of 16S rRNA genes. In the present study, DNA–DNA hybridization analysis revealed that C. absonum strains DSM 599T, DSM 600 and KZ 1544 shared 83·0–86·3 % DNA relatedness with C. sardiniense DSM 2632T. 16S rRNA gene sequence analysis showed that the C. absonum strains also shared high identity with C. sardiniense DSM 2632T (99·7, 99·3 and 99·8 % for DSM 599T, DSM 600 and KZ 1544, respectively), implying that C. absonum and C. sardiniense are synonyms. In addition, alignment of the inferred amino acid sequences for phospholipase C (PLC) indicated 96·5 % identity between PLCs from C. sardiniense and C. absonum, but relatively low identity with other clostridial species. These results strongly suggest that the species C. sardiniense and C. absonum should be united, with the name C. sardiniense having priority.

Abbreviations: PLC, phospholipase C

Published online ahead of print on 4 January 2005 as DOI 10.1099/ijs.0.63271-0.

The GenBank/EMBL/DDBJ accession numbers for the sequence of the gene encoding phospholipase C of C. sardiniense DSM 2632T and for the 16S rRNA gene sequences of C. sardiniense DSM 2632T and C. absonum (DSM 599T, DSM 600 and KZ 1544) are AB162962, AB161367–AB161368 and AB161369–AB161374, respectively.

Results of DNA–DNA hybridizations are available as supplementary material in IJSEM Online.

{dagger}Present address: Department of Medicine, Infectious Diseases Section, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73190, USA.

{ddagger}Present address: JCS Inc., 5-758 Nakamozu-cho, Sakai 591-8023, Japan.

§Present address: Department of Laboratory Sciences, School of Health Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan.






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