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Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)

¹ Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062, USA
² Laboratory Services, British Columbia Centre for Disease Control, Vancouver BC, Canada V5Z 4R4
³ Avenir Group, Inserm U570, Avenir Group, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France
⁴ Mycobacteria/Nocardia Laboratory, University of Texas Health Center, Tyler, TX 75708, USA
⁵ Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
⁶ Office of Laboratory Animal Care, University of California, Berkeley, CA 94720, USA
⁷ Department of Comparative Medicine, Stanford University, Stanford, CA 94305, USA
⁸ MIDI, Inc. Research and Development, Newark, DE 19713, USA
⁹ US Geological Survey, Leetown Science Center, National Fish Health Research Laboratory, Kearneysville, WV 25430, USA

Correspondence
Martha W. Rhodes
martha{at}vims.edu

A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass (Morone saxatilis) during an epizootic of mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus Mycobacterium. Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of Mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein (erp) and the gene encoding the 65 kDa heat-shock protein (hsp65) and restriction enzyme analysis of the hsp65 gene demonstrated that this group of isolates is unique. Insertion sequences associated with Mycobacterium ulcerans, IS2404 and IS2606, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 °C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 µg ml^–1), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, Mycobacterium pseudoshottsii sp. nov. The type strain, L15^T, has been deposited in the American Type Culture Collection as ATCC BAA-883^T and the National Collection of Type Cultures (UK) as NCTC 13318^T.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and hsp65 gene sequences of Mycobacterium pseudoshottsii ATCC BAA-883^T are AY570988 and AY571788, respectively.

Aligned erp gene sequences of Mycobacterium pseudoshottsii sp. nov., Mycobacterium marinum, Mycobacterium shottsii, Mycobacterium ulcerans and Mycobacterium tuberculosis are available as a supplementary figure in IJSEM Online.

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