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Pseudomonas argentinensis sp. nov., a novel yellow pigment-producing bacterial species, isolated from rhizospheric soil in Córdoba, Argentina

Alvaro Peix^1,, Odile Berge², Raúl Rivas³, Adriana Abril⁴ and Encarna Velázquez³

¹ Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain
² CEA/Cadarache, DSV-DEVM-LEMIR, Laboratoire d'Écologie Microbienne de la Rhizosphère, UMR6191 CNRS-CEA-Univ, Mediterranée, F-13108 Saint-Paul-Lez-Durance, France
³ Departamento de Microbiología y Génetica, Universidad de Salamanca, Spain
⁴ Microbiología Agrícola, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Argentina

Correspondence
Alvaro Peix
alvarp{at}usal.es

During a study in the Argentinian region of Chaco (Córdoba), some strains were isolated from the rhizosphere of grasses growing in semi-desertic arid soils. Two of these strains, one isolated from the rhizospheric soil of Chloris ciliata (strain CH01^T) and the other from Pappophorum caespitosum (strain PA01), were Gram-negative, strictly aerobic rods, which formed yellow round colonies on nutrient agar. They produced a water-insoluble yellow pigment, and a fluorescent pigment was also detected. A polyphasic taxonomic approach was used to characterize the strains. Comparison of the 16S rRNA gene sequences showed a similarity of 99·3 % between them, and phylogenetic analysis revealed that the strains belong to the genus Pseudomonas, within the -subclass of the Proteobacteria. The closest related species is Pseudomonas straminea IAM 1598^T (similarity of 99·0 % to strain CH01^T and 98·8 % to strain PA01), clustering in a separate branch with the various methods of tree building used. Strains CH01^T and PA01 both had a single polar flagellum, like other yellow pigment-producing pseudomonads related to them. Both strains produced catalase and oxidase. Similar to P. straminea, they did not hydrolyse gelatin or casein. The G+C DNA contents determined were 57·5 mol% for CH01^T and 58·0 mol% for PA01. DNA–DNA hybridization results showed 81 % relatedness between them, and only 40–44 % relatedness with respect to the type strain of P. straminea. These results, together with other phenotypic characteristics, support the conclusion that both isolates belong to the same species, and should be described as representing a novel species within the genus Pseudomonas, for which the name Pseudomonas argentinensis sp. nov. is proposed. The type strain is CH01^T (=LMG 22563^T=CECT 7010^T).

Published online ahead of print on 23 December 2004 as DOI 10.1099/ijs.0.63445-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains CH01^T and PA01 are AY691188 and AY691189, respectively.

A phylogenetic tree for Pseudomonas argentinensis sp. nov. and related species of the genus Pseudomonas sensu stricto, rep-PCR patterns, similarity matrix data and reciprocal values for percentage DNA–DNA hybridization are available as supplementary material in IJSEM Online.

Present address: UMR 1229 INRA-Université, de Bourgogne ‘Microbiologie et Géochimie des Sols’, INRA, 17 Rue Sully, BP 86510, 21065 Dijon CEDEX, France.

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