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Int J Syst Evol Microbiol 55 (2005), 1101-1106; DOI  10.1099/ijs.0.63485-0
© 2005 International Union of Microbiological Societies

Mycobacterium florentinum sp. nov., isolated from humans

Enrico Tortoli1, Laura Rindi2, Khye S. Goh3, Marja L. Katila4, Alessandro Mariottini1,5, Romano Mattei6, Gianna Mazzarelli1,7, Sini Suomalainen8, Pirjo Torkko4,9 and Nalin Rastogi3

1 Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, 50134 Florence, Italy
2 Department of Experimental Pathology, Medical Biotechnologies, Infectivology and Epidemiology, University of Pisa, 56127 Pisa, Italy
3 Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, 97165 Pointe-à-Pitre, Guadeloupe, France
4 Department of Clinical Microbiology, Kuopio University Hospital, 70211 Kuopio, Finland
5 Genetics and Cytogenetics Unit, Careggi Hospital, 50134 Florence, Italy
6 Clinical Laboratory, Campo di Marte Hospital, 55100 Lucca, Italy
7 Microbiological and Virological Serum-immunology Laboratory, Careggi Hospital, 50134 Florence, Italy
8 Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland
9 Laboratory of Environmental Microbiology, National Public Health Institute, 70211 Kuopio, Finland

Correspondence
Enrico Tortoli
e.tortoli{at}libero.it

Eight mycobacterial strains isolated during an 11 year period from the sputum of independent patients with various pulmonary disorders and, in one case, from a lymph node of a young girl, were found to present identical features. Phenotypic and genotypic characteristics revealed that the most closely related species to these test isolates were Mycobacterium triplex and Mycobacterium lentiflavum. However, the lipids of the cell wall of the test isolates differed from those of the latter species by TLC and presented unique profiles by both GC and HPLC. Genotypic analysis showed that they had unique 16S rRNA gene and internal transcribed spacer (ITS) sequences, and could be differentiated from all other mycobacterial strains by PCR restriction analysis of hsp65. The strains presented high resistance to antimycobacterial drugs. The name Mycobacterium florentinum sp. nov. is proposed for this taxon, with strain FI-93171T (=DSM 44852T=CIP 108409T) as the type strain.

Abbreviations: ITS, internal transcribed spacer; PRA, PCR restriction analysis

Published online ahead of print on 9 December 2004 as DOI 10.1099/ijs.0.63485-0.

The GenBank/EMBL/DDBJ accession number of 16S rRNA gene and ITS region sequence of strain FI-93171T is AJ616230.

Antimicrobial susceptibility data for three strains of M. florentinum are available as supplementary material in IJSEM Online.






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