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Candida digboiensis sp. nov., a novel anamorphic yeast species from an acidic tar sludge-contaminated oilfield

¹ Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector 39A, Chandigarh 160036, India
² TERI School of Advanced Studies, The Energy and Resources Institute (TERI), Darbari Seth Block, India Habitat Centre, Lodhi Road, New Delhi 110003, India
³ TERI, Darbari Seth Block, India Habitat Centre, Lodhi Road, New Delhi 110003, India

Correspondence
G. S. Prasad
prasad{at}imtech.res.in

Two strains (TERI-6^T and TERI-7) of a novel yeast species were isolated from acidic tar sludge-contaminated soil samples collected from Digboi Refinery, Assam, India. These two yeast strains were morphologically, physiologically and phylogenetically identical to each other. No sexual reproduction was observed on corn meal, malt, Gorodkowa, YM or V8 agars. Physiologically, the novel isolates were most closely related to Candida blankii, but differed in eight physiological tests. The prominent differences were the ability of the isolates to assimilate melibiose and inulin and their inability to assimilate D-glucuronate, succinate and citrate. Phylogenetic analysis using the D1/D2 variable domain showed that the closest relative of these strains is C. blankii (2·8 % divergence). Other related species are Zygoascus hellenicus and Candida bituminiphila. The isolates differed from C. blankii by 11 base substitutions in the 18S rRNA gene sequence and by 58 base substitutions in the internal transcribed spacer sequences. The physiological, biochemical and molecular data support the contention that strains TERI-6^T and TERI-7 represent a novel species, for which the name Candida digboiensis sp. nov. is proposed. The type strain is TERI-6^T (=MTCC 4371^T=CBS 9800^T=JCM 12300^T).

Published online ahead of print on 29 October 2004 as DOI 10.1099/ijs.0.63313-0.

The GenBank/EMBL/DDBJ accession numbers for the 26S rRNA gene D1/D2 domain, ITS and 18S rRNA gene sequences of strains MTCC 4371^T and MTCC 4372 are respectively AJ549212 and AJ549213, AJ697745 and AJ697746, and AJ697749 and AJ697750, and the accession numbers for the ITS sequences of C. blankii MTCC 1442^T and MTCC 624 are AJ697747 and AJ697748.

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